Anti-ZAP-70, clone 2F3.2, FITC,(mouse monoclonal IgG2a )

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	FC, ICC, WB	M	FITC	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R

FC, ICC, WB

FITC

Monoclonal Antibody

Description
Catalogue Number	16-210
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate
Overview	Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals Product Description: Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC) Immunogen: GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 corresponding to amino acids 1-254
Alternate Names	70 kDa zeta-associated protein Syk-related tyrosine kinase, zeta-chain (TCR) associated protein kinase (70 kD) zeta-chain (TCR) associated protein kinase 70kDa zeta-chain associated protein kinase 70kDa zeta-chain associated protein kinase, 70kD
Background Information	ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.

Description

Catalogue Number

16-210

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate

Overview

Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals

Product Description: Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC)

Immunogen: GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 corresponding to amino acids 1-254

Alternate Names

70 kDa zeta-associated protein
Syk-related tyrosine kinase, zeta-chain (TCR) associated protein kinase (70 kD)
zeta-chain (TCR) associated protein kinase 70kDa
zeta-chain associated protein kinase 70kDa
zeta-chain associated protein kinase, 70kD

Background Information

ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.

Product Information
Format	FITC
Control	Western Blot: Jurkat cell lysate.
Presentation	Mouse monoclonal FITC-conjugated IgG2a in buffer containing PBS containing 0.05% sodium azide. Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC). Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.

Product Information

Format

FITC

Control

Western Blot: Jurkat cell lysate.

Presentation

Mouse monoclonal FITC-conjugated IgG2a in buffer containing PBS containing 0.05% sodium azide.

Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC). Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.

Applications
Application	Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate detects level of ZAP-70 & has been published & validated for use in FC, IC & WB.
Key Applications	Flow Cytometry Immunocytochemistry Western Blotting
Application Notes	Immunocytochemistry: 5-20 µg/mL of a previous lot detected ZAP-70 in Jurkat cells fixed with formaldehyde and permeabilized with saponin.

Applications

Application

Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate detects level of ZAP-70 & has been published & validated for use in FC, IC & WB.

Key Applications

Flow Cytometry
Immunocytochemistry
Western Blotting

Application Notes

Immunocytochemistry:
5-20 µg/mL of a previous lot detected ZAP-70 in Jurkat cells fixed with formaldehyde and permeabilized with saponin.

Biological Information
Immunogen	GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 correspon-ding to amino acids 1-254. Clone 2F3.2.
Clone	clone 2F3.2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes human ZAP-70.
Isotype	IgG2a
Species Reactivity	Human Rat
Species Reactivity Note	Human. Expected to cross-react with rat.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001070
Entrez Gene Summary	This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Gene Symbol	ZAP70 ZAP-70 TZK STD SRK
Purification Method	Protein G Purified
UniProt Number	P43403
UniProt Summary	FUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation. SIZE: 619 amino acids; 69872 Da SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity). TISSUE SPECIFICITY: Expressed in T- and natural killer cells. DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z. PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity. DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
Molecular Weight	70 kDa

Biological Information

Immunogen

GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 correspon-ding to amino acids 1-254. Clone 2F3.2.

Clone

clone 2F3.2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Recognizes human ZAP-70.

Isotype

IgG2a

Species Reactivity

Human
Rat

Species Reactivity Note

Human. Expected to cross-react with rat.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_001070

Entrez Gene Summary

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Gene Symbol

ZAP70
ZAP-70
TZK
STD
SRK

Purification Method

Protein G Purified

UniProt Number

P43403

UniProt Summary

FUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
SIZE: 619 amino acids; 69872 Da
SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.

Molecular Weight

70 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on Jurkat lysates. Western Blot Analysis: 1:500 dilution of this lot detected ZAP 70 on 10 μg of Jurkat lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis:
1:500 dilution of this lot detected ZAP 70 on 10 μg of Jurkat lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 6 months at 2-8°C from date of receipt. For maximum recovery of product, centrifuge original vial prior to removing cap.

Storage and Shipping Information

Storage Conditions

Stable for 6 months at 2-8°C from date of receipt. For maximum recovery of product, centrifuge original vial prior to removing cap.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-ZAP-70, clone 2F3.2, FITC,(mouse monoclonal IgG2a )

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