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Anti-Ash2, clone 7E1 (rat monoclonal)

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货号：MABE72

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M IP, WB M Purified Monoclonal Antibody
Description
Catalogue Number MABE972
Description Anti-PRDM5 Antibody
Alternate Names
  • PRDM5
  • PR domain zinc finger protein 5
  • PR domain-containing protein 5
Background Information PR domain zinc finger protein 5 (UniProt Q9NQX1) is encoded by the PRDM1 gene (also known as BCS2, PFM2) in human (Gene ID 11107). PRDM proteins constitute a family of transcriptional regulators that are characterized by the presence of variable numbers of zinc finger domains and an N-terminal PR domain. PRDM5 can negatively regulate gene transcriptions by recruiting the G9a histone methyltransferase and histone deacetylases. PRDM5 can promote transcription by targeting enhancer-like elements or recruiting RNA polymerase II binding. PRDM5 is highly expressed in murine embryonic stem cells (mESCs) and associates with complexes involved in chromatin organization, such as Ctcf, Smc1 (cohesin), and TFIIIC, which indicates their involvement in chromatin organization. PRDM5 is often found silenced in human breast, ovarian, liver, and cervical cancers.
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-PRDM5 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of PRDM5 .
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application Notes Immunoprecipitation Analysis: A representative lot immunoprecipitated PRDM5 in mouse embryonic fibroblasts (Data courtesy of Dr. Anders H. Lund, University of Copenhagen, Denmark).
Immunoprecipitation: A representative lot of this antibody immunoprecipitated PRDMS E9 and E10 intrahepatic cholangiocarcinoma (ICC) cells. (Seehawer, M., et al. (2018). Nature 562(7725); 69-75).
Biological Information
Immunogen GST-tagged recombinant protein corresponding to human PRDM5 near the N-terminus.
Epitope Near N-terminus
Clone 57-20
Concentration Please refer to lot specific datasheet.
Host Mouse
Specificity Expected to react with all four spliced isoforms
Isotype IgG2bκ
Species Reactivity
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PRDM5
  • PFM2
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~73 kDa observed. 73.1 kDa (isoform 1, calculated), 69.9 kDa (isoform 2, calculated), 12.9 kDa (isoform 3, calculated), 58.1 kDa (isoform 4, calculated).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in mouse ESC lysate.

Western Blotting Analysis: 8.0 µg/mL of this antibody detected PRDM5 in 10 µg of mouse ESC lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 200 μg
Transport Information
Supplemental Information
Specifications

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