[关闭]
您好！欢迎来到炼石商城   请登录  注册    兼职小伙伴  
我的购物车
0
Qiagen 凯杰 Biosharp Omega

首页抗体Millipore 密理博>

图片仅供参考，请以实物为准
收藏

Anti-USP7, clone 1G7 (mouse monoclonal)

市场价：
0.00
促销价：
¥0.00

货号：05-1946

品牌：Millipore 密理博

配送至：

支付： 账期 货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

客服 郭恒 蔡玉坤 曾宪飞 技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次
RNeasy Mini Kit (50) 50次
3720.00
Plasmid Mini Kit I(200) 200次
Plasmid Mini Kit I(200) 200...
576.00
10ul吸头，透明，袋装，可灭菌 1000支/袋，50袋/箱
10ul吸头，透明，袋装，可灭菌...
85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (10) -10
QIAfilter Plasmid Maxi Kit (...
3000.00
QIAamp DNA Blood Midi Kit (20) 20次
QIAamp DNA Blood Midi Kit (2...
2680.00
QIAamp UCP Pathogen Mini Kit (50)
QIAamp UCP Pathogen Mini Kit...
4112.00
Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IP, IHC M Purified Monoclonal Antibody
Description
Catalogue Number 05-1946
Description Anti-USP7 Antibody, clone 1G7
Alternate Names
  • ubiquitin specific peptidase 7 (herpes virus-associated)
  • Ubiquitin-specific-processing protease 7
  • ubiquitin specific protease 7 (herpes virus-associated)
  • Herpesvirus-associated ubiquitin-specific protease
  • Herpes virus-associated ubiquitin-specific protease
  • Ubiquitin thioesterase 7
  • ubiquitin specific peptidase 7
  • Deubiquitinating enzyme 7
  • HAUSP
Background Information Ubiquitin carboxyl-terminal hydrolase 7 (USP7) is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates.[Saridakis, 2005] Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, Ubiquitin carboxyl-terminal hydrolase 7 activity generally stabilizes its substrate proteins. Ubiquitin carboxyl-terminal hydrolase 7 is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.[Canning, 2004]
References
Product Information
Format Purified
Control
  • HeLa cell lysate
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-USP7 Antibody, clone 1G7 is validated for use in WB, IP, IH for the detection of USP7.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry
Biological Information
Immunogen Histadine-tagged recombinant protein corresponding to human USP7.
Clone 1G7
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody recognizes USP7.
Isotype IgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • USP7
  • TEF1
  • HAUSP
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCITON: Cleaves ubiquitin fusion protein substrates. Deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and also induces TP53-dependent cell growth repression and apoptosis.

CATALYTIC ACTIVITY: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.

ENZYME REGULATION: Inhibited by N-ethyl-maleimide (NEM) and divalent cations. Tolerates high concentrations of NaCl but is inhibited at concentrations of 195 mM and higher. Ref.7

SUBUNIT STRUCTURE: Monomer. Interacts with TP53, MDM2 and UBXN6. Interacts with herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 and Epstein-Barr virus EBNA1. EBNA1 shows a 10-fold higher affinity than TP53 and can compete with it for USP7 binding. Binding to VMW110 may modulate the substrate specificity or activity of USP7 to stabilize viral proteins. Interacts with ATXN1 and the strength of interaction is influenced by the length of the poly-Gln region in ATXN1. A weaker interaction seen with mutants having longer poly-Gln regions.

SUBCELLULAR LOCATION: Nucleus. Note: Present in a minority of ND10 nuclear bodies. Association with VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus.

TISSUE SPECIFICTY: Widely expressed.

PTM: Polyneddylated (By similarity).

Polyubiquitinated (By similarity).

SEQUENCE SIMILARITIES: Belongs to the peptidase C19 family.

Contains 1 MATH domain.

BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Active from pH 7.0 to 9.5.
Molecular Weight ~ 128kDa Observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HeLa lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected USP7 on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！

我要咨询

咨询内容：
购物指南 免费注册 批量采购 购物流程 购物保障
配送方式 上门自提 第三方配送 炼石配送
支付方式 货到付款 账期付款
售后服务 售后政策 退款说明 售后流程 免责条款

服务热线

0771-3293894

关于我们|帮助中心|诚聘英才|炼石云实验室|联系我们|友情链接

Copyright 2014-2025 炼石云商网络科技（海南）有限公司版权所有 琼ICP备2023006525号-3