Anti-PARP-2, clone 4G8 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, ELISA, IP, IHC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

WB, ELISA, IP, IHC

Purified

Monoclonal Antibody

Description
Catalogue Number	MABE18
Description	Anti-PARP-2 Antibody, clone 4G8
Alternate Names	Poly [ADP-ribose] polymerase 1 PARP-1 NAD(+) ADP-ribosyltransferase 1 ADPRT 1 Poly[ADP-ribose] synthase 1 ARTD1
Background Information	Poly(ADP-ribose) polymerase (PARP) is a highly conserved nuclear enzyme present in higher eukaryotes. This gene is a member of a family of enzymes that synthesize and transfer ADP-ribose polymers to protein to modify their functional properties. A key role of PARP in the cell is mediating the repair of single-strand DNA nicks. PARP-2 forms homodimers and hetero dimers with PARP-1 in the base excision repair (BER) pathway. To mediate repair PARP interacts with DNA and recruits DNA repair protein XRCC1, DNA ligase III, DNA polymerase beta and a kinase to the site of damage. As a marker for apoptosis, it has been shown that PARP cleavage mediated by caspase-3 activity occurs at the onset of apoptosis, and inhibition of PARP cleavage attenuates apoptosis in vitro.

Description

Catalogue Number

MABE18

Description

Anti-PARP-2 Antibody, clone 4G8

Alternate Names

Poly [ADP-ribose] polymerase 1
PARP-1
NAD(+) ADP-ribosyltransferase 1
ADPRT 1
Poly[ADP-ribose] synthase 1
ARTD1

Background Information

Poly(ADP-ribose) polymerase (PARP) is a highly conserved nuclear enzyme present in higher eukaryotes. This gene is a member of a family of enzymes that synthesize and transfer ADP-ribose polymers to protein to modify their functional properties. A key role of PARP in the cell is mediating the repair of single-strand DNA nicks. PARP-2 forms homodimers and hetero dimers with PARP-1 in the base excision repair (BER) pathway. To mediate repair PARP interacts with DNA and recruits DNA repair protein XRCC1, DNA ligase III, DNA polymerase beta and a kinase to the site of damage. As a marker for apoptosis, it has been shown that PARP cleavage mediated by caspase-3 activity occurs at the onset of apoptosis, and inhibition of PARP cleavage attenuates apoptosis in vitro.

Product Information
Format	Purified
Control	HeLa cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

HeLa cell lysate

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-PARP-2 Antibody, clone 4G8 is a Mouse Monoclonal Antibody for detection of PARP-2 also known as poly (ADP-ribose) polymerase 2 or poly(ADP-ribose) synthetase & has been validated in WB, ELISA, IP & IHC.
Key Applications	Western Blotting ELISA Immunoprecipitation Immunohistochemistry
Application Notes	Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Maeda, Y., et al. (2006). JBC. 281(14):9600-9606.) Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Department of Biochemistry, Molecular Biology, and Immunology. jyelamos@um.es) ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (von Kobbe, C., et al. (2004). Nucleic Acids Research. 32(13):4003-4014.)

Applications

Application

Anti-PARP-2 Antibody, clone 4G8 is a Mouse Monoclonal Antibody for detection of PARP-2 also known as poly (ADP-ribose) polymerase 2 or poly(ADP-ribose) synthetase & has been validated in WB, ELISA, IP & IHC.

Key Applications

Western Blotting
ELISA
Immunoprecipitation
Immunohistochemistry

Application Notes

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Maeda, Y., et al. (2006). JBC. 281(14):9600-9606.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Department of Biochemistry, Molecular Biology, and Immunology. jyelamos@um.es)

ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (von Kobbe, C., et al. (2004). Nucleic Acids Research. 32(13):4003-4014.)

Biological Information
Immunogen	Recombinant protein corresponding to mouse PARP-2.
Epitope	Unknown
Clone	4G8
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes PARP-2.
Isotype	IgG1κ
Species Reactivity	Human Mouse
Species Reactivity Note	Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_033762
Entrez Gene Summary	This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. (provided by RefSeq)
Gene Symbol	PARP-2 hPARP-2 ADPRT2 ADPRTL2 ADPRTL3 pADPRT-2
Purification Method	Protein G Purified
UniProt Number	O88554
UniProt Summary	FUNCTION: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. CATALYTIC ACTIVITY: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. SUBUNIT STRUCTURE: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP1, POLB and LIG3. Homo- and heterodimer with PARP1. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Widely expressed; the highest levels were in testis followed by ovary. Expression is correlated with proliferation, with higher levels occurring during early fetal development and organogenesis and in the highly proliferative cell compartments of adult. DEVELOPMENTAL STAGE: At stage E12.5, expressed at high level in the developing liver and kidneys. At E18.5, preferentially expressed in the thymus and in regions of the nervous system. Within the developing trunk, preferential expression persisted in the liver and became restricted to the cortical region of the kidney, spleen, adrenal gland, and to stomach and intestinal epithelia. From E14.5 to E18.5, as well as in the adult, expressed at the highest level in thymus. Expression is particularly high in the subcapsular zone of the thymus where immature lymphocytes proliferate. INDUCTION: By high levels of DNA-damaging agents. PTM: Poly-ADP-ribosylated by PARP1. Acetylation reduces DNA binding and enzymatic activity. SEQUENCE SIMILARITIES: Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. SEQUENCE CAUTION: The sequence AAC25415.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight	Calculated molecular weight is 66 kDa. Typically observed at 62 kDa on western blots.

Biological Information

Immunogen

Recombinant protein corresponding to mouse PARP-2.

Epitope

Unknown

Clone

4G8

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes PARP-2.

Isotype

IgG1κ

Species Reactivity

Human
Mouse

Species Reactivity Note

Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_033762

Entrez Gene Summary

This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of
catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly
(ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain
of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential
DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two
alternatively spliced transcript variants encoding distinct isoforms have been found. (provided by RefSeq)

Gene Symbol

PARP-2
hPARP-2
ADPRT2
ADPRTL2
ADPRTL3
pADPRT-2

Purification Method

Protein G Purified

UniProt Number

O88554

UniProt Summary

FUNCTION: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.

CATALYTIC ACTIVITY: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.

SUBUNIT STRUCTURE: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP1, POLB and LIG3. Homo- and heterodimer with PARP1.

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY: Widely expressed; the highest levels were in testis followed by ovary. Expression is correlated with proliferation, with higher levels occurring during early fetal development and organogenesis and in the highly proliferative cell compartments of adult.

DEVELOPMENTAL STAGE: At stage E12.5, expressed at high level in the developing liver and kidneys. At E18.5, preferentially expressed in the thymus and in regions of the nervous system. Within the developing trunk, preferential expression persisted in the liver and became restricted to the cortical region of the kidney, spleen, adrenal gland, and to stomach and intestinal epithelia. From E14.5 to E18.5, as well as in the adult, expressed at the highest level in thymus. Expression is particularly high in the subcapsular zone of the thymus where immature lymphocytes proliferate.

INDUCTION: By high levels of DNA-damaging agents.

PTM: Poly-ADP-ribosylated by PARP1.

Acetylation reduces DNA binding and enzymatic activity.

SEQUENCE SIMILARITIES: Contains 1 PARP alpha-helical domain.

Contains 1 PARP catalytic domain.

SEQUENCE CAUTION: The sequence AAC25415.1 differs from that shown. Reason: Erroneous initiation.

Molecular Weight

Calculated molecular weight is 66 kDa. Typically observed at 62 kDa on western blots.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PARP-2 on 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PARP-2 on 10 µg of HeLa cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-PARP-2, clone 4G8 (mouse monoclonal)

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