Anti-Notch 1, extracellular (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	WB, IH(P)	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R

WB, IH(P)

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	ABS90
Description	Anti-Notch 1 Antibody, extracellular
Alternate Names	Notch homolog 1, translocation-associated (Drosophila) Notch (Drosophila) homolog 1 (translocation-associated) Translocation-associated notch protein TAN-1 neurogenic locus notch homolog protein 1
Background Information	The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch-1. notch-2, notch-3, and notch-4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of notch-1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

Description

Catalogue Number

ABS90

Description

Anti-Notch 1 Antibody, extracellular

Alternate Names

Notch homolog 1, translocation-associated (Drosophila)
Notch (Drosophila) homolog 1 (translocation-associated)
Translocation-associated notch protein TAN-1
neurogenic locus notch homolog protein 1

Background Information

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch-1. notch-2, notch-3, and notch-4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of notch-1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

Product Information
Format	Affinity Purified
Control	Human lymph node lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Human lymph node lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Applications
Application	Anti-Notch 1 Antibody, extracellular detects level of Notch 1 & has been published & validated for use in WB, IH(P).
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Immunohistochemistry Analysis: 1:400 dilution of this antibody has been shown to detect Notch 1 in gastrointestinal and colorectal carcinoma tissue.

Applications

Application

Anti-Notch 1 Antibody, extracellular detects level of Notch 1 & has been published & validated for use in WB, IH(P).

Key Applications

Western Blotting
Immunohistochemistry (Paraffin)

Application Notes

Immunohistochemistry Analysis: 1:400 dilution of this antibody has been shown to detect Notch 1 in gastrointestinal and colorectal carcinoma tissue.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the extracellular domain of rat Notch 1.
Epitope	Extracellular domain
Host	Rabbit
Specificity	This antibody recognizes Notch 1.
Species Reactivity	Human Rat
Species Reactivity Note	Demonstrated to react with human and rat. Expected to react with mouse (97% sequence homology), chimpanzee (92% sequence homology) and canine (91% sequence homology).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001099191
Entrez Gene Summary	Transmembrane receptor; involved in cell-cell interactions important for development and pattern formation [RGD].
Gene Symbol	TAN1 notch1 hN1 Notch 1
Purification Method	Affinity Purfied
UniProt Number	Q07008
UniProt Summary	FUNCTION: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity). Acts instructively to control the cell fate determination of CNS multipotent progenitor cells, resulting in astroglial induction and neuron/oligodendrocyte suppression. SUBUNIT STRUCTURE: Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1 (By similarity). SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). Notch 1 intracellular domain: Nucleus (By similarity). Note: Following proteolytical processing NICD is translocated to the nucleus (By similarity). TISSUE SPECIFICITY: Expressed in the brain, kidney and spleen. Expressed in postnatal central nervous system (CNS) germinal zones and, in early postnatal life, within numerous cells throughout the CNS. Found in both subventricular and ventricular germinal zones. DEVELOPMENTAL STAGE: In the embryo, highest levels occur between days 12 and 14 and decrease rapidly to much lower levels in the adult. PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). Phosphorylated (By similarity). O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose (By similarity). SEQUENCE SIMILARITIES: Belongs to the NOTCH family. Contains 5 ANK repeats. Contains 36 EGF-like domains. Contains 3 LNR (Lin/Notch) repeats.
Molecular Weight	~260 kDa observed. Notch 1, extracellular is calculated at ~183 kDa.

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to the extracellular domain of rat Notch 1.

Epitope

Extracellular domain

Host

Rabbit

Specificity

This antibody recognizes Notch 1.

Species Reactivity

Human
Rat

Species Reactivity Note

Demonstrated to react with human and rat. Expected to react with mouse (97% sequence homology), chimpanzee (92% sequence homology) and canine (91% sequence homology).

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_001099191

Entrez Gene Summary

Transmembrane receptor; involved in cell-cell interactions important for development and pattern formation [RGD].

Gene Symbol

TAN1
notch1
hN1
Notch 1

Purification Method

Affinity Purfied

UniProt Number

Q07008

UniProt Summary

FUNCTION: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity). Acts instructively to control the cell fate determination of CNS multipotent progenitor cells, resulting in astroglial induction and neuron/oligodendrocyte suppression.

SUBUNIT STRUCTURE: Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1 (By similarity).

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity).

Notch 1 intracellular domain: Nucleus (By similarity). Note: Following proteolytical processing NICD is translocated to the nucleus (By similarity).

TISSUE SPECIFICITY: Expressed in the brain, kidney and spleen. Expressed in postnatal central nervous system (CNS) germinal zones and, in early postnatal life, within numerous cells throughout the CNS. Found in both subventricular and ventricular germinal zones.

DEVELOPMENTAL STAGE: In the embryo, highest levels occur between days 12 and 14 and decrease rapidly to much lower levels in the adult.

PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).

Phosphorylated (By similarity).

O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose (By similarity).

SEQUENCE SIMILARITIES: Belongs to the NOTCH family.

Contains 5 ANK repeats.

Contains 36 EGF-like domains.

Contains 3 LNR (Lin/Notch) repeats.

Molecular Weight

~260 kDa observed. Notch 1, extracellular is calculated at ~183 kDa.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in human lymph node lysate. Western Blot Analysis: 1:500 dilution of this antibody detected Notch 1 on 10 µg of human lymph node lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in human lymph node lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected Notch 1 on 10 µg of human lymph node lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Notch 1, extracellular (rabbit polyclonal)

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