Anti-MT1-MMP, catalytic domain (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Chp	WB, ICC, IHC, IP	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, Chp

WB, ICC, IHC, IP

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB6005
Replaces	AB8102
Description	Anti-MT1-MMP Antibody, catalytic domain
Alternate Names	MT1MMP MMP-14 MMP14 Membrane-type-1 matrix metalloproteinase Membrane-type matrix metalloproteinase 1 matrix metalloproteinase 14 (membrane-inserted) matrix metallopeptidase 14 (membrane-inserted)
Background Information	Matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes degrade components of extracellular matrix (ECM), including collagens, fibronectin, laminin and other glycoproteins. MMPs play an important role in wound healing, apoptosis, development, angiogenesis, cancer metastases, and tissue remodeling. Most MMPs are secreted as inactive proproteins which are cleaved and activated; however, MT1-MMP (MMP-14) is a member of the membrane-type subfamily. Each member of this subfamily has a transmembrane domain suggesting these proteins are expressed at the cell’s surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of ECM components and thought to be an important tool for cellular remodeling of the ECM. It is also involved in endothelial cell migration, and although not fully characterized, MT1-MMP activity appears to modulate endothelial invasion, and formation of capillary tubes during angiogenesis (Galvez, 2001). It also appears to be a factor in monocyte recruitment during inflammation (Matias-Roman, 2005). MT1-MMP also activates MMP-2 protein, an activity involved in tumor invasion.

Description

Catalogue Number

AB6005

Replaces

AB8102

Description

Anti-MT1-MMP Antibody, catalytic domain

Alternate Names

MT1MMP
MMP-14
MMP14
Membrane-type-1 matrix metalloproteinase
Membrane-type matrix metalloproteinase 1
matrix metalloproteinase 14 (membrane-inserted)
matrix metallopeptidase 14 (membrane-inserted)

Background Information

Matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes degrade components of extracellular matrix (ECM), including collagens, fibronectin, laminin and other glycoproteins. MMPs play an important role in wound healing, apoptosis, development, angiogenesis, cancer metastases, and tissue remodeling. Most MMPs are secreted as inactive proproteins which are cleaved and activated; however, MT1-MMP (MMP-14) is a member of the membrane-type subfamily. Each member of this subfamily has a transmembrane domain suggesting these proteins are expressed at the cell’s surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of ECM components and thought to be an important tool for cellular remodeling of the ECM. It is also involved in endothelial cell migration, and although not fully characterized, MT1-MMP activity appears to modulate endothelial invasion, and formation of capillary tubes during angiogenesis (Galvez, 2001). It also appears to be a factor in monocyte recruitment during inflammation (Matias-Roman, 2005). MT1-MMP also activates MMP-2 protein, an activity involved in tumor invasion.

Product Information
Format	Affinity Purified
Control	HT-1080 cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

HT-1080 cell lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-MT1-MMP Antibody, catalytic domain is an antibody against MT1-MMP for use in WB, IC, IH & IP.
Key Applications	Western Blotting Immunocytochemistry Immunohistochemistry Immunoprecipitation
Application Notes	Western Blot Analysis: 0.5 µg/mL from a previous lot detected MT1-MMP in 10 µg of human normal uterus tissue lysate. Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected MT1-MMP in prostate carcinoma tissue. Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated MT1-MMP from 500 µg of human normal uterus RIPA lysate.

Applications

Application

Anti-MT1-MMP Antibody, catalytic domain is an antibody against MT1-MMP for use in WB, IC, IH & IP.

Key Applications

Western Blotting
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation

Application Notes

Western Blot Analysis: 0.5 µg/mL from a previous lot detected MT1-MMP in 10 µg of human normal uterus tissue lysate.

Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected MT1-MMP in prostate carcinoma tissue.

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated MT1-MMP from 500 µg of human normal uterus RIPA lysate.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the catalytic domain of human MT1-MMP.
Epitope	Catalytic domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	The antibody recognizes MT1-MMP (MMP-14).
Species Reactivity	Human Chimpanzee
Species Reactivity Note	Demonstrated to react with human. Predicted to react with chimpanzee based on 100% sequence homology. Equine, sheep, porcine, canine, and bovine (99% sequence homology). Mouse and rat (97% sequence homology). Orangutan (92% sequence homology).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_004986.1
Entrez Gene Summary	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq].
Gene Symbol	MMP14 MTMMP1 MMP-14 MT1MMP MT1-MMP MMP-X1
Purification Method	Affinity Purfied
UniProt Number	P50281
UniProt Summary	FUNCTION: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. COFACTOR: Binds 1 zinc ion per subunit (by similarity). & Calcium (by similarity). SIZE: 582 amino acids; 65884 Da SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck. DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
Molecular Weight	~ 65 kDa

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to the catalytic domain of human MT1-MMP.

Epitope

Catalytic domain

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

The antibody recognizes MT1-MMP (MMP-14).

Species Reactivity

Human
Chimpanzee

Species Reactivity Note

Demonstrated to react with human. Predicted to react with chimpanzee based on 100% sequence homology.
Equine, sheep, porcine, canine, and bovine (99% sequence homology). Mouse and rat (97% sequence homology). Orangutan (92% sequence homology).

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_004986.1

Entrez Gene Summary

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq].

Gene Symbol

MMP14
MTMMP1
MMP-14
MT1MMP
MT1-MMP
MMP-X1

Purification Method

Affinity Purfied

UniProt Number

P50281

UniProt Summary

FUNCTION: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.

COFACTOR: Binds 1 zinc ion per subunit (by similarity). & Calcium (by similarity).

SIZE: 582 amino acids; 65884 Da

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.

DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.

Molecular Weight

~ 65 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HT-1080 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected MT1-MMP in 10 µg of HT-1080 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HT-1080 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MT1-MMP in 10 µg of HT-1080 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-MT1-MMP, catalytic domain (rabbit polyclonal)

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