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Anti-Paxillin (N-Term) (rabbit monoclonal IgG1)

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货号：04-581

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Rb IP, WB, IHC Rb Purified Polyclonal Antibody
Description
Catalogue Number AB3794
Replaces 04-581
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Paxillin Antibody
References
Product Information
Format Purified
Presentation Purified immunoglobulin. Liquid in PBS with 50% Glycerol and 0.01% sodium azide, pH 7.3.
Applications
Application Detect Paxillin using this Anti-Paxillin Antibody validated for use in IP, WB, IH.
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry
Application Notes Western blot: 1:1,000 on mouse brain lysates.
Immunohistochemistry: 1:10.
Immunoprecipitation: 5 μL/mL lysate

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Recombinant human paxillin.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Paxillin
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Rabbit
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PXN
  • Paxillin.
  • paxillin
  • FLJ16691
UniProt Number
UniProt Summary FUNCTION: SwissProt: P49023 # Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
SIZE: 591 amino acids; 64533 Da
SUBUNIT: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.
PTM: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
SIMILARITY: SwissProt: P49023 ## Belongs to the paxillin family. & Contains 4 LIM zinc-binding domains.
Molecular Weight 68 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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