Anti-EGF Receptor (neutralizing),clone LA1, Alexa Fluor® 555 Conjugate (mouse monoclonal IgG1)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	ELISA, IP, WB, ICC, IH(P)	M	Ascites	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

ELISA, IP, WB, ICC, IH(P)

Ascites

Monoclonal Antibody

Description
Catalogue Number	MAB2170
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Huntingtin Protein Antibody, a.a. 1247-1646, clone HU-4E6

Description

Catalogue Number

MAB2170

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Huntingtin Protein Antibody, a.a. 1247-1646, clone HU-4E6

Product Information
Format	Ascites
Presentation	Ascites fluid. Liquid, does not contain any preservative.

Product Information

Format

Ascites

Presentation

Ascites fluid. Liquid, does not contain any preservative.

Applications
Application	Detect Huntingtin Protein using this Anti-Huntingtin Protein Antibody, a.a. 1247-1646, clone HU-4E6 validated for use in ELISA, IP, WB, IC, IH(P).
Key Applications	ELISA Immunoprecipitation Western Blotting Immunocytochemistry Immunohistochemistry (Paraffin)
Application Notes	ELISA: 1:500-1:5,000 Western blot (recombinant protein, not suitable for the natural Huntingtin) : 1:500-1:5,000 Immunohistochemistry on frozen and microwave oven treated paraffin sections (human): 1:500-1:5,000 Immunocytochemistry on transfected cells: 1:500-1:5,000 Immunoprecipitation: 1:500-1:5,000 Optimal working dilutions must be determined by the end user.

Applications

Application

Detect Huntingtin Protein using this Anti-Huntingtin Protein Antibody, a.a. 1247-1646, clone HU-4E6 validated for use in ELISA, IP, WB, IC, IH(P).

Key Applications

ELISA
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)

Application Notes

ELISA: 1:500-1:5,000

Western blot (recombinant protein, not suitable for the natural Huntingtin) : 1:500-1:5,000

Immunohistochemistry on frozen and microwave oven treated paraffin sections (human): 1:500-1:5,000

Immunocytochemistry on transfected cells: 1:500-1:5,000

Immunoprecipitation: 1:500-1:5,000

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Huntingtin fragment from aa 1247 to 1646 as a fusion protein
Epitope	a.a. 1247-1646
Clone	HU-4E6
Host	Mouse
Specificity	Huntingtin Protein. No detectable cross reactivity with other proteins by Western blot.
Isotype	IgG2b
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_002111.6
Entrez Gene Summary	Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression.
Gene Symbol	HTT IT15 SLC6A4 Huntingtin OCD1 HD
UniProt Number	P42858
UniProt Summary	FUNCTION: SwissProt: P42858 # May play a role in microtubule-mediated transport or vesicle function. SIZE: 3144 amino acids; 347860 Da SUBUNIT: Binds SH3GLB1 (By similarity). Interacts through its N- terminus with FNBP3. Interacts with PQBP1, SETD2 and SYVN. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICITY: Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. PTM: Cleaved by apopain downstream of the polyglutamine stretch. The resulting amino-terminal fragment is cytotoxic and provokes apoptosis. & Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. DISEASE: SwissProt: P42858 # Defects in HD are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum). SIMILARITY: SwissProt: P42858 ## Belongs to the hungtintin family. & Contains 10 HEAT repeats.

Biological Information

Immunogen

Huntingtin fragment from aa 1247 to 1646 as a fusion protein

Epitope

a.a. 1247-1646

Clone

HU-4E6

Host

Mouse

Specificity

Huntingtin Protein. No detectable cross reactivity with other proteins by Western blot.

Isotype

IgG2b

Species Reactivity

Human
Mouse

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_002111.6

Entrez Gene Summary

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression.

Gene Symbol

HTT
IT15
SLC6A4
Huntingtin
OCD1
HD

UniProt Number

P42858

UniProt Summary

FUNCTION: SwissProt: P42858 # May play a role in microtubule-mediated transport or vesicle function.
SIZE: 3144 amino acids; 347860 Da
SUBUNIT: Binds SH3GLB1 (By similarity). Interacts through its N- terminus with FNBP3. Interacts with PQBP1, SETD2 and SYVN.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
PTM: Cleaved by apopain downstream of the polyglutamine stretch. The resulting amino-terminal fragment is cytotoxic and provokes apoptosis. & Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
DISEASE: SwissProt: P42858 # Defects in HD are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
SIMILARITY: SwissProt: P42858 ## Belongs to the hungtintin family. & Contains 10 HEAT repeats.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C in undiluted aliquots up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-EGF Receptor (neutralizing),clone LA1, Alexa Fluor® 555 Conjugate (mouse monoclonal IgG1)

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