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Anti-Pras40, Clone 73P21

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货号：05-988

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IP, WB M Purified Monoclonal Antibody
Description
Catalogue Number 05-988
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Pras40 Antibody, Clone 73P21
Alternate Names
  • 40 kDa proline-rich AKT substrate
  • AKT1 substrate 1 (proline-rich)
  • proline-rich Akt substrate, 40 kDa
Background Information PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
References
Product Information
Format Purified
Control
  • RIPA lysates of HeLa cells.
Presentation Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.1% sodium azide.
Applications
Application Anti-Pras40 Antibody, Clone 73P21 is an antibody against Pras40 for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application Notes Immunoblot Analysis: A 01-1.0 μg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
Biological Information
Immunogen Recombinant human PRAS40 (full-length) expressed in E. coli.
Clone 73P21
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Recognizes PRAS40.
Isotype IgG1
Species Reactivity
  • Human
Species Reactivity Note Human. This antibody does not cross react with mouse.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]
Gene Symbol
  • AKT1S1
  • Lobe
  • MGC2865
  • PRAS40
Purification Method Affinity Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q96B36 # May play an important role in phosphatidylinositol 3- kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
SIZE: 256 amino acids; 27383 Da
SUBUNIT: The phosphorylated form interacts with 14-3-3.
SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity). Note=Found in the cytosolic fraction of the brain (By similarity).
TISSUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
Molecular Weight 40 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by western blot.

Western Blot Analysis:
A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 years at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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