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Anti-PLD, clone SKB2 (mouse monoclonal IgG)

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号:05-608

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IP, WB M Purified Monoclonal Antibody
Description
Catalogue Number 05-608
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-PLD Antibody, clone SKB2
References
Product Information
Format Purified
Presentation 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide before the addition of glycerol to 30%
Applications
Application Detect PLD using this Anti-PLD Antibody, clone SKB2 validated for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Biological Information
Immunogen GST fusion-protein corresponding to residues 326-504 of human PLD 1
Clone clone SKB2
Host Mouse
Specificity PLD 1 and PLD 2
Isotype IgG
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis (Hammond et al., 1995 [PubMed 8530346]).[supplied by OMIM]
Gene Symbol
  • PLD1
  • hPLD1
Purification Method Protein G Chromatography
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q13393 # Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).
SIZE: 1074 amino acids; 124184 Da
SUBUNIT: Interacts with PIP5K1A.
SUBCELLULAR LOCATION: Cytoplasm, perinuclear region (By similarity). Endoplasmic reticulum (By similarity). Golgi apparatus (By similarity). Late endosome (By similarity).
TISSUE SPECIFICITY: Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
SIMILARITY: SwissProt: Q13393 ## Belongs to the phospholipase D family. & Contains 1 PH domain. & Contains 2 PLD phosphodiesterase domains. & Contains 1 PX (phox homology) domain.
Molecular Weight 116 kDa (PLD1), 100 kDa (PLD2)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on RIPA lysates from HeLa cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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