Anti-BrdU, clone BU-1 (mouse culture supernatant)

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Anti-BrdU, clone BU-1 (mouse culture supernatant)

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货号：05-633

品牌：Millipore 密理博

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Species Reactivity	Key Applications	Host	Format	Antibody Type
A	ICC	M	Culture Supernatant	Monoclonal Antibody

Description
Catalogue Number	05-633
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-BrdU Antibody, clone BU-1
Background Information	The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

References

Product Information
Format	Culture Supernatant
Presentation	4 vials, each vial containing mouse culture supernatant lyophilized from 2.5 mL. The supernatant contains mycoplasma, which contribute enzymes that facilitate antibody binding. Lyophilized powder.

Applications
Application	Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC.
Key Applications	Immunocytochemistry

Biological Information
Immunogen	5-iodouridine:ovalbumin conjugate
Clone	clone BU-1
Host	Mouse
Specificity	5-Bromo-2-deoxyuridine
Isotype	IgG2a
Species Reactivity	All
Antibody Type	Monoclonal Antibody
Purification Method	Concentrated culture supernatant
Molecular Weight	none given

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	A representative lot was evaluated by immunocytochemistry on HeLa cells. A 1:2-1:4 dilution from a representative lot showed positive immunostaining for Anti-BrdU in HeLa cells fixed with 95% ethanol, 5% glacial acetic acid. This lot was tested using the DNA Replication Assay Kit (Catalog # 17-316). Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

A representative lot was evaluated by immunocytochemistry on HeLa cells. A 1:2-1:4 dilution from a representative lot showed positive immunostaining for Anti-BrdU in HeLa cells fixed with 95% ethanol, 5% glacial acetic acid. This lot was tested using the DNA Replication Assay Kit (Catalog # 17-316).

Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	2 years at -20°C

Packaging Information
Material Size	4 vials

Transport Information

Supplemental Information

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Anti-BrdU, clone BU-1 (mouse culture supernatant)

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