Anti-b-Tubulin, clone AA2

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Anti-b-Tubulin, clone AA2

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货号：05-661

品牌：Millipore 密理博

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北京朝阳

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Replacement Information

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M, B	WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-661
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-β-Tubulin Antibody, clone AA2
Alternate Names	Tubulin beta-5 chain beta 5-tubulin beta Ib tubulin beta-4 tubulin tubulin beta polypeptide tubulin beta-1 chain tubulin, beta tubulin, beta polypeptide
Background Information	Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each about 55 kDa in size. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.

References

Product Information
Format	Purified
Control	Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
Presentation	Purified mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide and 30% glycerol.

Applications
Application	This Anti-β-Tubulin Antibody, clone AA2 is validated for use in WB for the detection of β-Tubulin.
Key Applications	Western Blotting

Biological Information
Immunogen	Purified bovine brain tubulin. The epitope recognized by this antibody has been mapped to amino acids 412-430.
Epitope	The epitope has been mapped to amino acids 412-430
Clone	AA2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes β-Tubulin isotypes I, II, III, IVa, and IVb of ~50 kDa. No cross-reactivity with alpha or gamma tubulin.
Isotype	IgG1
Species Reactivity	Human Rat Mouse Bovine
Species Reactivity Note	Proven to react with human, rat, bovine, and mouse. Predicted to have broad species cross-reactivity based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_178014
Gene Symbol	M40 MGC117247 MGC16435 OK/SW-cl.56 OTTHUMP00000029069 TUBB1 TUBB5 Tubb5
Purification Method	Protein G Purified
UniProt Number	P07437
UniProt Summary	FUNCTION: SwissProt: P07437 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE: 444 amino acids; 49671 Da SUBUNIT: Dimer of alpha and beta chains. TISSUE SPECIFICITY: Ubiquitously expressed with highest levels in spleen, thymus and immature brain. DOMAIN: SwissProt: P07437 The highly acidic C-terminal region may bind cations such as calcium. SIMILARITY: Belongs to the tubulin family.
Molecular Weight	50 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by western blot on RIPA lysates from 3T3/A31, PC-12, Jurkat, or A431 cells. Western Blot Analysis: 0.1-1.0 µg/mL of this lot detected β-Tubulin in RIPA lysates from 3T3/A31, PC-12, Jurkat, and A431 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage