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Anti-G9a (rabbit polyclonal IgG)

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货号：07-551

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB Rb Purified Polyclonal Antibody
Description
Catalogue Number 07-551
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-G9a Antibody
Alternate Names
  • KMT1C
Background Information Protein G9a (also known as Histone H3-K9 methyltransferase 3, Euchromatic histone-lysine N-methyltransferase 2 and HLA-B-associated transcript 8)
References
Product Information
Format Purified
Control
  • Positive Control Included: HeLa Nuclear Extract (12-309).
Presentation Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Applications
Application Use Anti-G9a Antibody (Rabbit Polyclonal Antibody) validated in WB to detect G9a also known as KMT1C.
Key Applications
  • Western Blotting
Biological Information
Immunogen Peptide (C-DERVDSDSKSEVEALTEQ) corresponding to amino acids 71-88 of human G9a.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes human G9a protein.
Isotype IgG
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described.
Gene Symbol
  • EHMT2
  • NG36/G9a
  • NG36
  • G9A
  • Em:AF134726.3
  • C6orf30
  • BAT8
  • FLJ35547
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q96KQ7 # Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1 (By similarity).
SIZE: 1210 amino acids; 132370 Da
SUBUNIT: Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1.
SUBCELLULAR LOCATION: Nucleus. Note=Associates with euchromatic regions. Does not associate with heterochromatin.
TISSUE SPECIFICITY: Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
DOMAIN:SwissProt: Q96KQ7 The SET domain mediates interaction with WIZ.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. & Contains 7 ANK repeats. & Contains 1 post-SET domain. & Contains 1 pre-SET domain. & Contains 1 SET domain.
Molecular Weight 140 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot HeLa nuclear extract cell lysate
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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