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Anti-MAP2, clone 5F9

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货号：05-346

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Rb WB, IHC M Purified Monoclonal Antibody
Description
Catalogue Number 05-346
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-MAP2 Antibody, clone 5F9
References
Product Information
Format Purified
Presentation 200 µg of protein G purified mouse IgG1 in 200 µL of storage buffer (0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide. Frozen at -20°C.
Applications
Application Detect MAP2 using this Anti-MAP2 Antibody, clone 5F9 validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Biological Information
Immunogen Purified rat brain microtubules MAP2
Clone clone 5F9
Host Mouse
Specificity MAP2
Isotype IgG1
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Rabbit
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The MAP2 gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
Gene Symbol
  • MAP2
  • MAP2A
  • MAP2B
Purification Method Protein G Chromatography
UniProt Number
UniProt Summary FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
SIZE: 1,827 amino acids (Isofoim 1; MAP2b); 471 amino acids (Isofoim 2; MAP2c); 1,823 amino acids (Isofoim 3; MAP2a); 559 amino acids (Isofoim 4; MAP2d);
SUBCELLULAR LOCATION: Cytoplasm. Cytoskeleton.
PTM: Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Isoform 2/MAP2c is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
Molecular Weight 300kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated on rat brain microsomal preparation (Catalog #12-144).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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