Anti-E2F-1, clones KH20 and KH95 (mixed mouse monoclonal IgGs) - 炼石商城

您好！欢迎来到炼石商城请登录注册兼职小伙伴

我的购物车

0

Qiagen 凯杰 Biosharp Omega

全部商品分类

小伙伴店铺

客服热线 0771-3293894

首页抗体Millipore 密理博>

图片仅供参考，请以实物为准

收藏

Anti-E2F-1, clones KH20 and KH95 (mixed mouse monoclonal IgGs)

市场价：: 0.00

促销价：: ¥0.00

货号：05-379

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次

RNeasy Mini Kit (50) 50次

3720.00

Plasmid Mini Kit I(200) 200次

Plasmid Mini Kit I(200) 200...

576.00

10ul吸头，透明，袋装，可灭菌 1000支/袋，50袋/箱

10ul吸头，透明，袋装，可灭菌...

85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (10) -10

QIAfilter Plasmid Maxi Kit (...

3000.00

QIAamp DNA Blood Midi Kit (20) 20次

QIAamp DNA Blood Midi Kit (2...

2680.00

QIAamp UCP Pathogen Mini Kit (50)

QIAamp UCP Pathogen Mini Kit...

4112.00

商品介绍
商品咨询

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Xn	IP, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-379
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-E2F-1 Antibody, clones KH20 and KH95
Background Information	E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

References

Product Information
Format	Purified
Control	Positive Control Included: HeLa Nuclear Extract (12-309)
Presentation	Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide.

Applications
Application	Anti-E2F-1 Antibody, clones KH20 & KH95 is a high quality Mouse Monoclonal Antibody for the detection of E2F-1 & has been validated in IP & WB.
Key Applications	Immunoprecipitation Western Blotting

Biological Information
Immunogen	GST fusion protein corresponding to full-length human E2F-1.
Epitope	The epitope for KH20 has been mapped to amino acids 1-89; KH95 to amino acids 342-386.
Clone	KH20 & KH95
Host	Mouse
Specificity	Recognizes E2F-1.
Isotype	IgG
Species Reactivity	Human Xenopus
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_005225
Entrez Gene Summary	The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Gene Symbol	E2F1 RBAP-1 E2F-1 RBP3 RBBP3 RBBP-3 PBR3
Purification Method	Protein G Purified
UniProt Number	Q01094
UniProt Summary	FUNCTION: SwissProt: Q01094 # Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. SIZE: 437 amino acids; 46920 Da SUBUNIT: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T- antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A. SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. SIMILARITY: SwissProt: Q01094 ## Belongs to the E2F/DP family.
Molecular Weight	55 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Immunoblot Analysis: 0.5-2μg/ml of this antibody detected E2F-1 in a HeLa nuclear extract. Immunoprecipitation: 4μg of this antibody immunoprecipitated E2F-1 from HeLa nuclear extract. Gel Shift: The KH20 clone has been shown to gel shift the E2F-1/DP-1/DNA complex (Helin, 1993; Wu, 2000).
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Packaging Information
Material Size	200 µg

Transport Information

Supplemental Information

Specifications

登录 / 注册

购物指南免费注册批量采购购物流程购物保障

配送方式上门自提第三方配送炼石配送

支付方式货到付款账期付款

售后服务售后政策退款说明售后流程免责条款

服务热线

0771-3293894