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Anti-Histone H2A.Z

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货号：07-594

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB Rb Serum Polyclonal Antibody
Description
Catalogue Number 07-594
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Histone H2A.Z Antibody
References
Product Information
Format Serum
Presentation 0.05% sodium azide before the addition of glycerol to 30%
Applications
Application Detect Histone H2A.Z also known as H2AZ histone with Anti-Histone H2A.Z Antibody (Rabbit Polyclonal Antibody), that has been demonstrated to work in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen peptide containing the sequence SLIGKKGQQ, corres-ponding to the C-terminus of human histone H2A.Z
Host Rabbit
Specificity C-terminus of histone H2A.Z
Isotype IgG
Species Reactivity
  • Human
Species Reactivity Note Broad species cross-reactivity is expected
Antibody Type Polyclonal Antibody
Entrez Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.
Gene Symbol
  • H2AFZ
  • H2A/z
  • H2A.Z
  • MGC117173
  • H2AZ
  • H2A.z
Purification Method Serum
UniProt Number
UniProt Summary FUNCTION: SwissProt: P0C0S5 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
SIZE: 128 amino acids; 13553 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP (By similarity).
SUBCELLULAR LOCATION: Nucleus.
PTM: Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression. & Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis (By similarity). & Not phosphorylated (By similarity).
SIMILARITY: SwissProt: P0C0S5 ## Belongs to the histone H2A family.
Molecular Weight 16kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on acid extracted proteins from HeLa cells and recombinant Histone H2A.Z (Catalog #14-597), no cross-reactivity with Histone H2A
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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