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PDK1 (aa52-556)Active BULK SZ 250ug

市场价：
30.00
促销价：
¥0.00

货号：14-452M

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number 14-452M
Brand Family Upstate
Trade Name
  • Upstate
Description PDK1 Protein, active, 250 µg
Overview N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end.
References
Product Information
Presentation 50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200μM PMSF and 5% glycerol
Applications
Application Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Source Human PDK1 expressed by baculovirus in Sf21 insect cells
Specific Activity For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • PDPK1
  • MGC20087
  • PDK1
  • PkB-like
  • PRO0461
  • MGC35290
  • hPDK1
Protein Target PDK1
Purification Method Ni2+/NTA-agarose
Target Sub-Family AGC
UniProt Number
UniProt Summary FUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
SIZE: 556 amino acids; 63152 Da
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
TISSUE SPECIFICITY: Appears to be expressed ubiquitously.
PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
SIMILARITY: SwissProt: O15530 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight 59kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by phosphorylation of PDKtide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 6 months at -70°C
Packaging Information
Material Size 250 µg
Material Package Also available in 10 μg size (cat#14-452) and in bulk (cat# 14-452-K).
Transport Information
Supplemental Information
Specifications

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