Anti-Paxillin, clone 5H11

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Anti-Paxillin, clone 5H11

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货号：05-417

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M, B, Av	ICC, IP, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-417
Replaces	04-581
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Paxillin Antibody, clone 5H11
Alternate Names	FLJ16691 paxillin
Background Information	Paxillin, a focal adhesion scaffolding protein, is phosphorylated on multiple tyrosine residues in response various stimulations including integrin stimulation, growth factor stimulation, and oncogenic transformation (v-Src, v-Crk and BCR/ABL). Paxillin binds many other focal adhesion proteins including FAK, Vinculin, Talin, and Tensin. As such, Paxillin and it tyrosine phosphorylation, help regulate the dynamics of the focal adhesions and the actin cytoskeleton which results in cell morphology and cell migration characteristics of the cell. Paxillin is most notable phosphorylated on tyrosines 31 and 118 (Tyr31 and Tyr118). Interestingly, Vinculin and FAK bind to overlapping sites on Paxillin. Additionally, there is an increase in Paxillin phosphorylation at Tyr118 in vinculin (-/-) and Vinculin Y822F cells. Paxillin Y31F Y118F expression restores apoptosis and inhibits Erk activity.

References

Product Information
Format	Purified
Control	Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Presentation	Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Store at -20°C.

Applications
Application	Anti-Paxillin Antibody, clone 5H11 is an antibody against Paxillin for use in IC, IP & WB.
Key Applications	Immunocytochemistry Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation: 4 μg of a previous lot of antibody immunoprecipitated paxillin from 500 μg of a 3T3/A31 RIPA lysate. Immunocytochemistry: 5-10 μg/mL of a previous lot gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7% paraformaldehyde for 5 minutes.

Biological Information
Immunogen	GST fusion protein containing fulllength human paxillin. Clone 5H11.
Clone	5H11
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes paxillin.
Isotype	IgG1
Species Reactivity	Human Rat Mouse Bovine Avian
Species Reactivity Note	Human, mouse, rat, chicken and bovine. Other species cross-reactivity not tested.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_002859
Gene Symbol	PXN Paxillin paxillin FLJ16691
Purification Method	Protein G Purified
UniProt Number	P49023
UniProt Summary	FUNCTION: SwissProt: P49023 # Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). SIZE: 591 amino acids; 64533 Da SUBUNIT: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5. PTM: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. SIMILARITY: SwissProt: P49023 ## Belongs to the paxillin family. & Contains 4 LIM zinc-binding domains.
Molecular Weight	68 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on RIPA lysates from 3T3/A31 fibroblasts. Western Blot Analysis: 0.5-2 μg/mL of this lot detected paxillin in RIPA lysates from 3T3/A31 fibroblasts.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.