Anti-Cbl, clone 7G10 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	IP, WB, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

IP, WB, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	05-440
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Cbl Antibody, clone 7G10
Alternate Names	Cas-Br-M (murine) ecotropic retroviral transforming sequence Casitas B-lineage lymphoma proto-oncogene Proto-oncogene c-CBL RING finger protein 55 Signal transduction protein CBL oncogene CBL2
Background Information	Casitas B-lineage lymphoma (Cbl), is an oncogene which causes pre B-cell lymphomas. It participates in the regulation of cell signaling and has the ability to interact with many proteins such as receptor/non-receptor PTK's, structural proteins, and novel proteins that are not well understood. Diseases associated with Cbl are autoimmune diseases, diabetes, and leukemia. By modifying Cbl's ability to bind to oncogenic proteins, Cbl may be developed into an anti-cancer tool and in the near future used in therapeutic intervention.

Description

Catalogue Number

05-440

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Cbl Antibody, clone 7G10

Alternate Names

Cas-Br-M (murine) ecotropic retroviral transforming sequence
Casitas B-lineage lymphoma proto-oncogene
Proto-oncogene c-CBL
RING finger protein 55
Signal transduction protein CBL
oncogene CBL2

Background Information

Casitas B-lineage lymphoma (Cbl), is an oncogene which causes pre B-cell lymphomas. It participates in the regulation of cell signaling and has the ability to interact with many proteins such as receptor/non-receptor PTK's, structural proteins, and novel proteins that are not well understood. Diseases associated with Cbl are autoimmune diseases, diabetes, and leukemia. By modifying Cbl's ability to bind to oncogenic proteins, Cbl may be developed into an anti-cancer tool and in the near future used in therapeutic intervention.

Product Information
Format	Purified
Control	Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 μl of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20μg of reduced lysate per lane for minigels
Presentation	Purified IgG1k in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4

Product Information

Format

Purified

Control

Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 μl of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20μg of reduced lysate per lane for minigels

Presentation

Purified IgG1k in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4

Applications
Application	This Anti-Cbl Antibody, clone 7G10 is validated for use in IP, WB, IC for the detection of Cbl.
Key Applications	Immunoprecipitation Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry: 5-10 μg/mL of a previous lot showed positive immunostaining for Cbl in 3T3/A31 cells fixed with ice cold Methanol/Ethanol [1:1] for 1 minute. Immunoprecipitation: 2 μg of a previous lot immunoprecipitated Cbl from 1 mg of a mouse 3T3/A31 cell RIPA lysate, as reported by an independent laboratory.

Applications

Application

This Anti-Cbl Antibody, clone 7G10 is validated for use in IP, WB, IC for the detection of Cbl.

Key Applications

Immunoprecipitation
Western Blotting
Immunocytochemistry

Application Notes

Immunocytochemistry:
5-10 μg/mL of a previous lot showed positive immunostaining for Cbl in 3T3/A31 cells fixed with ice cold Methanol/Ethanol [1:1] for 1 minute.

Immunoprecipitation:
2 μg of a previous lot immunoprecipitated Cbl from 1 mg of a mouse 3T3/A31 cell RIPA lysate, as reported by an independent laboratory.

Biological Information
Immunogen	GST fusion protein corresponding to residues 878-906 of human Cbl
Epitope	a.a. 878-906
Clone	7G10
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Antibody recognizes amino acids 878-906 on human Cbl
Isotype	IgG1κ
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_005188
Entrez Gene Summary	The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.
Gene Symbol	CBL CBL2 RNF55 C-CBL
Purification Method	Protein G Chromatography
UniProt Number	P22681
UniProt Summary	FUNCTION: SwissProt: P22681 # Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. SIZE: 906 amino acids; 99647 Da SUBUNIT: Associates with NCK via its SH3 domain. The phosphorylated C-terminus interacts with CD2AP via its second SH3 domain. Binds to UBE2L3. Interacts with adapters SLA, SLA2 and with the phosphorylated C-terminus of SH2B2. Interacts with EGFR, SYK and ZAP70 via the highly conserved Cbl-N region. Also interacts with SORBS1 and INPPL1/SHIP2. Interacts with phosphorylated LAT2. May interact with CBLB (By similarity). SUBCELLULAR LOCATION: Cytoplasm. DOMAIN: SwissProt: P22681 The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. & The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain. PTM: Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.DISEASE:SwissProt: P22681 # Can be converted to an oncogenic protein by deletions or mutations that disturb its ability to down-regulate RTKs. SIMILARITY: Contains 1 CBL N-terminal domain. & Contains 2 EF-hand-like domains. & Contains 1 RING-type zinc finger. & Contains 1 SH2 domain. & Contains 1 UBA domain. MISCELLANEOUS: This protein has one functional calcium-binding site.
Molecular Weight	120kDa

Biological Information

Immunogen

GST fusion protein corresponding to residues 878-906 of human Cbl

Epitope

a.a. 878-906

Clone

7G10

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Antibody recognizes amino acids 878-906 on human Cbl

Isotype

IgG1κ

Species Reactivity

Human
Mouse
Rat

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_005188

Entrez Gene Summary

The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.

Gene Symbol

CBL
CBL2
RNF55
C-CBL

Purification Method

Protein G Chromatography

UniProt Number

P22681

UniProt Summary

FUNCTION: SwissProt: P22681 # Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
SIZE: 906 amino acids; 99647 Da
SUBUNIT: Associates with NCK via its SH3 domain. The phosphorylated C-terminus interacts with CD2AP via its second SH3 domain. Binds to UBE2L3. Interacts with adapters SLA, SLA2 and with the phosphorylated C-terminus of SH2B2. Interacts with EGFR, SYK and ZAP70 via the highly conserved Cbl-N region. Also interacts with SORBS1 and INPPL1/SHIP2. Interacts with phosphorylated LAT2. May interact with CBLB (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
DOMAIN: SwissProt: P22681 The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. & The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
PTM: Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.DISEASE:SwissProt: P22681 # Can be converted to an oncogenic protein by deletions or mutations that disturb its ability to down-regulate RTKs.
SIMILARITY: Contains 1 CBL N-terminal domain. & Contains 2 EF-hand-like domains. & Contains 1 RING-type zinc finger. & Contains 1 SH2 domain. & Contains 1 UBA domain.
MISCELLANEOUS: This protein has one functional calcium-binding site.

Molecular Weight

120kDa

Product Usage Statements
Quality Assurance	routinely evaluated by immunoblot on RIPA lysates from human Jurkat, mouse 3T3/A31, TF-1, or FDC-P1/Mac11
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

routinely evaluated by immunoblot on RIPA lysates from human Jurkat, mouse 3T3/A31, TF-1, or FDC-P1/Mac11

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8ºC from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Cbl, clone 7G10 (mouse monoclonal)

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