Anti-erbB-3/HER-3, clone H3.105.5

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	IP, NEUT	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

IP, NEUT

Purified

Monoclonal Antibody

Description
Catalogue Number	05-471
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-erbB-3/HER-3 Antibody, clone H3.105.5
Alternate Names	Tyrosine kinase-type cell surface receptor HER3 lethal congenital contracture syndrome 2 v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
Background Information	erbB3 (HER3, c-erbB3) is a receptor tyrosine kinase (RTK). erbB3 is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. The ErbB family of receptors are a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Though erbB3 is membrane bound and can bind its ligand, it does not have an active kinase domain so it can not transmit this signal without first forming a heterodimer with other EGFR family members. This heterodimerization then leads to proliferation and differentiation. erbB3 is also known to bind Phosphoinositide 3-kinase (PI3 Kinase, PI3K) though six tyrosine phosphorylation sites that PI3K can bind to, which results in the activation of Akt/PKB. erbB3 is known to be amplified in numerous cancers including breast and prostate.

Description

Catalogue Number

05-471

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-erbB-3/HER-3 Antibody, clone H3.105.5

Alternate Names

Tyrosine kinase-type cell surface receptor HER3
lethal congenital contracture syndrome 2
v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Background Information

erbB3 (HER3, c-erbB3) is a receptor tyrosine kinase (RTK). erbB3 is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. The ErbB family of receptors are a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Though erbB3 is membrane bound and can bind its ligand, it does not have an active kinase domain so it can not transmit this signal without first forming a heterodimer with other EGFR family members. This heterodimerization then leads to proliferation and differentiation. erbB3 is also known to bind Phosphoinositide 3-kinase (PI3 Kinase, PI3K) though six tyrosine phosphorylation sites that PI3K can bind to, which results in the activation of Akt/PKB. erbB3 is known to be amplified in numerous cancers including breast and prostate.

Product Information
Format	Purified
Control	Widely expressed.
Presentation	Purified mouse monoclonal IgG1 in buffer containing 10 mM PBS, pH 7.4. Frozen solution.

Product Information

Format

Purified

Control

Widely expressed.

Presentation

Purified mouse monoclonal IgG1 in buffer containing 10 mM PBS, pH 7.4. Frozen solution.

Applications
Application	Anti-erbB-3/HER-3 Antibody, clone H3.105.5 detects level of erbB-3/HER-3 & has been published & validated for use in IP, NEUT.
Key Applications	Immunoprecipitation Neutralizing
Application Notes	Immunoprecipitation: Reported to immunoprecipitate unoccupied erbB-3/HER-3 receptors.

Applications

Application

Anti-erbB-3/HER-3 Antibody, clone H3.105.5 detects level of erbB-3/HER-3 & has been published & validated for use in IP, NEUT.

Key Applications

Immunoprecipitation
Neutralizing

Application Notes

Immunoprecipitation:
Reported to immunoprecipitate unoccupied erbB-3/HER-3 receptors.

Biological Information
Immunogen	Extracellular domain of recombinant human erbB-3/HER-3 oncoprotein. Clone H3.105.5 Antibody Class IgG1, antibody produced by P3X63Ag8U1 (P3U1) x Balb/c spleen cells. Purified by ammonium sulfate precipitation, Sephacryl S-300 gel filtration and DEAE-52 chromatography.
Clone	H3.105.5
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes erbB-3, Mr 185 kDa, but not EGF receptor, erbB-2/HER-2, or erbB-4/HER-4 Antibody Class IgG1, antibody produced by P3X63Ag8U1 (P3U1) x Balb/c spleen cells. Purified by ammonium sulfate precipitation, Sephacryl S-300 gel filtration and DEAE-52 chromatography.
Isotype	IgG1
Species Reactivity	Human Mouse
Species Reactivity Note	Human and mouse, other species not tested.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001982
Entrez Gene Summary	This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
Gene Symbol	ERBB3 c-erbB-3 c-erbB3 erbB-3 p180-ErbB3 p85-sErbB3 p45-sErbB3 ErbB-3 erbB3-S MGC88033 HER3 MDA-BF-1
Purification Method	Protein G Purified
UniProt Number	P21860
UniProt Summary	FUNCTION: SwissProt: P21860 # Binds and is activated by neuregulins and NTAK. SIZE: 1342 amino acids; 148098 Da SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 and PA2G4. SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. TISSUE SPECIFICITY: Epithelial tissues and brain. DOMAIN: SwissProt: P21860 The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. PTM: Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (By similarity). DISEASE: SwissProt: P21860 # Overexpressed in a subset of human mammary tumors. & Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
Molecular Weight	185 kDa

Biological Information

Immunogen

Extracellular domain of recombinant human erbB-3/HER-3 oncoprotein. Clone H3.105.5

Antibody Class
IgG1, antibody produced by P3X63Ag8U1 (P3U1) x Balb/c spleen cells. Purified by ammonium sulfate precipitation, Sephacryl S-300 gel filtration and DEAE-52 chromatography.

Clone

H3.105.5

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes erbB-3, Mr 185 kDa, but not EGF receptor, erbB-2/HER-2, or erbB-4/HER-4
Antibody Class
IgG1, antibody produced by P3X63Ag8U1 (P3U1) x Balb/c spleen cells. Purified by ammonium sulfate precipitation, Sephacryl S-300 gel filtration and DEAE-52 chromatography.

Isotype

IgG1

Species Reactivity

Human
Mouse

Species Reactivity Note

Human and mouse, other species not tested.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_001982

Entrez Gene Summary

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

Gene Symbol

ERBB3
c-erbB-3
c-erbB3
erbB-3
p180-ErbB3
p85-sErbB3
p45-sErbB3
ErbB-3
erbB3-S
MGC88033
HER3
MDA-BF-1

Purification Method

Protein G Purified

UniProt Number

P21860

UniProt Summary

FUNCTION: SwissProt: P21860 # Binds and is activated by neuregulins and NTAK.
SIZE: 1342 amino acids; 148098 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 and PA2G4.
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Epithelial tissues and brain.
DOMAIN: SwissProt: P21860 The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
PTM: Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (By similarity).
DISEASE: SwissProt: P21860 # Overexpressed in a subset of human mammary tumors. & Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.

Molecular Weight

185 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by receptor neutralization. Receptor neutralization assay: 10 µg/mL of this lot blocked heregulin mediated activation of the erbB-3/HER-3 receptor in MCF-7 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by receptor neutralization.

Receptor neutralization assay:
10 µg/mL of this lot blocked heregulin mediated activation of the erbB-3/HER-3 receptor in MCF-7 cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-erbB-3/HER-3, clone H3.105.5

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