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Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R)

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货号：17-127

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Analytes Available Detection Methods
PA Ser/Thr Kinases Colorimetric
Description
Catalogue Number 17-127
Brand Family Upstate
Trade Name
  • Upstate
Description Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R)
References
Product Information
Components
  • Malachite Green Solution A (Cat.# 20-105)
  • Malachite Green Additive (Cat.# 20-104)
  • Phosphate Standard (Cat.# 20-103)
  • Threonine Phosphopeptide (K-R-pT-I-R-R) (Cat.# 12-219)
  • pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
  • pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
  • NiCl2, 40mM (Cat.# 20-178)
  • 96-well microtiter plate
Detection method Colorimetric
Applications
Application Ser/Thr Phosphatase Assay Kit 1used to detect PP2A activity by either dephosphorylation of the phosphopeptide.
Key Applications
  • Phosphatase Assay
Biological Information
Analytes Available
  • Ser/Thr Kinases
Entrez Gene Number
Entrez Gene Summary This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Defects in this gene could be the cause of some lung and colon cancers. At least two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • PPP2R1B
  • PP2A-A-beta
  • MGC26454
  • PR65B
UniProt Number
UniProt Summary FUNCTION: SwissProt: P30154 # The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
SIZE: 601 amino acids; 66214 Da
SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9.
DOMAIN: SwissProt: P30154 Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
DISEASE:SwissProt: P30154 # Defects in PPP2R1B might be a cause of some lung and colorectal cancers.
SIMILARITY: Belongs to the phosphatase 2A regulatory subunit A family. & Contains 15 HEAT repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely used to detect PP2A (Catalog # 14-111) activity by either dephosphorylation of the phosphopeptide (K-R-pT-I-R-R) or hydrolysis of pNPP
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 1 kit
Material Package Kit capacity: 100 assays
Transport Information
Supplemental Information
Specifications

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