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Qiagen 凯杰 Biosharp Omega

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Ras Activation Assay Kit (non-radioactive)

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货号：17-218

品牌：Millipore 密理博

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重要规格表

Key Applications
Enzyme Assays
Description
Catalogue Number 17-218
Replaces SGT435
Brand Family Upstate
Trade Name
  • Upstate
Description Ras Activation Assay Kit
References
Product Information
Components
  • Ras Assay Reagent (Raf-1 RBD, agarose) (Cat.# 14-278)
  • Anti-Ras, clone RAS10 (Cat.# 05-516)
  • 100X GTPγS, 10mM (Cat.# 20-176)
  • 100X GDP, 100mM (Cat.# 20-177)
  • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
Applications
Key Applications
  • Enzyme Assays
Biological Information
Entrez Gene Number
Entrez Gene Summary Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM]
Gene Symbol
  • MRAS
  • M-RAs
  • M-RAS
  • FLJ42964
  • R-RAS3
  • RRAS3
UniProt Number
UniProt Summary FUNCTION: SwissProt: O14807 # May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
SIZE: 208 amino acids; 23846 Da
SUBUNIT: Interacts with RGL3 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
TISSUE SPECIFICITY: Expression highly restricted to the brain and heart.
SIMILARITY: SwissProt: O14807 ## Belongs to the small GTPase superfamily. Ras family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance This kit precipitated Ras-GTP from HeLa cell lysates that had been loaded with GTPγS. The precipitated Ras-GTP was detected by western immunoblot using 1μg/ml anti-Ras, clone RAS10
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 1 kit
Material Package Kit capacity: 30 assays
Transport Information
Supplemental Information
Specifications

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