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Qiagen 凯杰 Biosharp Omega

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Rac2 Activation Assay Kit (non-radioactive)

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货号：17-369

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications
ABA
Description
Catalogue Number 17-369
Brand Family Upstate
Trade Name
  • Upstate
Description Rac2 Activation Assay Kit
References
Product Information
Components
  • Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
  • Anti-Rac2 (Cat.# 07-604)
  • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
  • 100X GTPγS, 10mM (Cat.# 20-176)
  • 100X GDP, 100mM (Cat.# 20-177)
Applications
Application Non-radioactive Rac2 Activation Assay Kit can be used to precipitate Rac2-GTP from cell lysates & detection by a Rac2 specific antibody.
Key Applications
  • Affinity Binding Assay
Biological Information
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases.
Gene Symbol
  • RAC2
  • p21-Rac2
  • GX
  • Gx
  • EN-7
  • HSPC022
UniProt Number
UniProt Summary FUNCTION: SwissProt: P15153 # Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Seems to be involved in the regulation of the NADPH oxidase.
SIZE: 192 amino acids; 21429 Da
SUBUNIT: Interacts with DOCK2, which may activate it.
SUBCELLULAR LOCATION: Cytoplasm. Note=Membrane-associated when activated.
TISSUE SPECIFICITY: Hematopoietic specific.
DISEASE:SwissProt: P15153 # Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome [MIM:608203].
SIMILARITY: SwissProt: P15153 ## Belongs to the small GTPase superfamily. Rho family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by Immunoprecipitation of Rac2-GTP from HL-60 cells that had been loaded with GTPγS.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 30 assays
Material Package Kit capacity: 30 assays
Transport Information
Supplemental Information
Specifications

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