Anti-Sortilin (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, IHC	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

WB, IHC

Serum

Polyclonal Antibody

Description
Catalogue Number	AB9712
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Sortilin Antibody
Alternate Names	Neurotensin receptor 3
Background Information	Neurotensin receptor 3 or sortilin (the intracellular sorting protein 95 kDa) is a member of a family of receptors characterized by a single transmembrane domain, a short intracytoplasmic domain, and a long cysteine-rich luminal extracellular domain. This receptor has been found in the brain, spinal cord, muscle and other peripheral cell types, and in cancer cells. Sortilin exhibits sequence similarities with proteins involved in intracellular transport and trafficking.

Description

Catalogue Number

AB9712

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Sortilin Antibody

Alternate Names

Neurotensin receptor 3

Background Information

Neurotensin receptor 3 or sortilin (the intracellular sorting protein 95 kDa) is a member of a family of receptors characterized by a single transmembrane domain, a short intracytoplasmic domain, and a long cysteine-rich luminal extracellular domain. This receptor has been found in the brain, spinal cord, muscle and other peripheral cell types, and in cancer cells. Sortilin exhibits sequence similarities with proteins involved in intracellular transport and trafficking.

Product Information
Format	Serum
Presentation	Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material.

Product Information

Format

Serum

Presentation

Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material.

Applications
Application	Anti-Sortilin Antibody is an antibody against Sortilin for use in WB, IH.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Western blot: 1:1,000-1:2,000 on extracts from NSC34 cells. Immunohistochemistry: 1:500-1:1,000 on rat spinal cord. Optimal working dilutions must be determined by the end user.

Applications

Application

Anti-Sortilin Antibody is an antibody against Sortilin for use in WB, IH.

Key Applications

Western Blotting
Immunohistochemistry

Application Notes

Western blot: 1:1,000-1:2,000 on extracts from NSC34 cells.
Immunohistochemistry: 1:500-1:1,000 on rat spinal cord.

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Recombinant protein from the extracellular domain of human sortilin.
Host	Rabbit
Specificity	Sortilin (Neurotensin receptor 3). By Western blot the antibody recognizes a strong band at ~90 kDa in extracts of NSC34 cells. By immunohistochemistry the antibody intensely stains motor neurons within the rat spinal cord.
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_002959.4
Entrez Gene Summary	This gene encodes a protein that is a multi-ligand type-1 receptor with similarity to the yeast carboxypeptidase Y sorting receptor Vps10 protein. The encoded protein, a trans-Golgi network (TGN) transmembrane protein, binds a number of unrelated ligands that participate in a wide range of cellular processes; however, it lacks the typical features of a signalling receptor. In the TGN, furin mediates the activation of the mature binding form. The encoded protein consists of a large luminal domain, a single transmembrane segment and short C-terminal cytoplasmic tail. The luminal domain contains a cysteine-rich region similar to two corresponding segments in the yeast Vps10p; the cytoplasmic tail is similar to the corresponding segment of the cation-independent mannose 6-phosphate receptor and the tail also interacts with the VHS domains of GGA (Golgi-associated, gamma-adaptin homologous, ARF-interacting) proteins.
Gene Symbol	SORT1 NT3 Gp95 NTR3
Purification Method	Serum
UniProt Number	Q99523
UniProt Summary	FUNCTION: SwissProt: Q99523 # Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. SIZE: 831 amino acids; 92068 Da SUBUNIT: Interacts with LPL and SLC2A4 (By similarity). Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP, GM2A and PSAP. Forms a complex with NGFR which binds specifically to the precursor forms of NGFB (proNGFB) and BDNF (proBDNF). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Note=Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.TISSUE SPECIFICITY: Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver. DOMAIN: SwissProt: Q99523 The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N- terminal propeptide and the extracellular domain may also inhibit premature ligand binding. & The extracellular domain may be shed following protease cleavage in some cell types. PTM: The N-terminal propeptide is cleaved by furin and possibly other homologous proteases. & Contains 8 intrachain disulfide bonds. & N-glycosylated. SIMILARITY: Contains 9 BNR repeats.

Biological Information

Immunogen

Recombinant protein from the extracellular domain of human sortilin.

Host

Rabbit

Specificity

Sortilin (Neurotensin receptor 3). By Western blot the antibody recognizes a strong band at ~90 kDa in extracts of NSC34 cells. By immunohistochemistry the antibody intensely stains motor neurons within the rat spinal cord.

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_002959.4

Entrez Gene Summary

This gene encodes a protein that is a multi-ligand type-1 receptor with similarity to the yeast carboxypeptidase Y sorting receptor Vps10 protein. The encoded protein, a trans-Golgi network (TGN) transmembrane protein, binds a number of unrelated ligands that participate in a wide range of cellular processes; however, it lacks the typical features of a signalling receptor. In the TGN, furin mediates the activation of the mature binding form. The encoded protein consists of a large luminal domain, a single transmembrane segment and short C-terminal cytoplasmic tail. The luminal domain contains a cysteine-rich region similar to two corresponding segments in the yeast Vps10p; the cytoplasmic tail is similar to the corresponding segment of the cation-independent mannose 6-phosphate receptor and the tail also interacts with the VHS domains of GGA (Golgi-associated, gamma-adaptin homologous, ARF-interacting) proteins.

Gene Symbol

SORT1
NT3
Gp95
NTR3

Purification Method

Serum

UniProt Number

Q99523

UniProt Summary

FUNCTION: SwissProt: Q99523 # Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.
SIZE: 831 amino acids; 92068 Da
SUBUNIT: Interacts with LPL and SLC2A4 (By similarity). Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP, GM2A and PSAP. Forms a complex with NGFR which binds specifically to the precursor forms of NGFB (proNGFB) and BDNF (proBDNF).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Note=Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.TISSUE SPECIFICITY: Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver.
DOMAIN: SwissProt: Q99523 The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N- terminal propeptide and the extracellular domain may also inhibit premature ligand binding. & The extracellular domain may be shed following protease cleavage in some cell types.
PTM: The N-terminal propeptide is cleaved by furin and possibly other homologous proteases. & Contains 8 intrachain disulfide bonds. & N-glycosylated.
SIMILARITY: Contains 9 BNR repeats.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added 1:1 for additional stability.

Storage and Shipping Information

Storage Conditions

Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added 1:1 for additional stability.

Packaging Information
Material Size	50 µL

Packaging Information

Material Size

50 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Sortilin (rabbit polyclonal)

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