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PI 3 Kinase HTRF Assay, 384 wells

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货号：33-016

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number 33-016
Brand Family Upstate
Trade Name
  • HTRF
  • Upstate
Description PI 3-Kinase HTRF™ Assay; 385 wells
References
Product Information
Components
  • 4X Reaction Buffer
  • PIP2 (Substrate; 1mM stock)
  • Stop Reagent A
  • Stop Reagent B
  • DM A Detection Reagent
  • DM B Detection Reagent
  • DM C Detection Reagent
Detection method HTRF
Applications
Application The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 1 plate.
Key Applications
  • Kinase Assay
Biological Information
Species Reactivity
  • Human
  • Mouse
Analytes Available
  • PI 3-Kinase
Entrez Gene Number
Entrez Gene Summary Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Gene Symbol
  • PIK3CA
  • MGC142163
  • PI3K
  • MGC142161
  • p110-alpha
Target Sub-Family PI3K/PI4K
UniProt Number
UniProt Summary FUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 1 plate
Material Package Kit capacity: 384 wells
Transport Information
Supplemental Information
Specifications

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