c-Kit (V654A)

Description
Catalogue Number	14-733
Brand Family	Chemicon®
Trade Name	Chemicon
Description	c-Kit (V654A) Protein, active, 10 µg
Overview	N-terminal GST tagged, recombinant human cKit, amino acids 544 - end containing the V654A mutation. Co-ordinates encompass the cytoplasmic domain (544-976) according in Swiss Prot P10721. V654A is an acquired resistance mutation which has been found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance and rapid progession of GISTs. (Chen LL et al., Cancer Research, (2004); 64:5913-5919).

Description

Catalogue Number

14-733

Brand Family

Chemicon®

Trade Name

Chemicon

Description

c-Kit (V654A) Protein, active, 10 µg

Overview

N-terminal GST tagged, recombinant human cKit, amino acids 544 - end containing the V654A mutation. Co-ordinates encompass the cytoplasmic domain (544-976) according in Swiss Prot P10721. V654A is an acquired resistance mutation which has been found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance and rapid progession of GISTs. (Chen LL et al., Cancer Research, (2004); 64:5913-5919).

Applications
Application	V654A is an acquired resistance mutation found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance & rapid progession of GISTs.
Key Applications	Kinase Assay

Applications

Application

V654A is an acquired resistance mutation found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance & rapid progession of GISTs.

Key Applications

Kinase Assay

Biological Information
Source	recombinant protein expressed in Sf21 cells
Specific Activity	169 U/mg, where one unit of cKit (V654A) activity is defined as 1nmol phosphate incorporated into 250 μM GGMEDIYFEFMGGKKK per minute at 30 °C with a final ATP concentration of 100 μM.
Entrez Gene Number	NM_000222.2 NM_001093772.1
Entrez Gene Summary	This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	KIT C-Kit PBT CD117 SCFR c-kit
Protein Target	c-Kit
Purification Method	Purified using glutathione-agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.
Target Sub-Family	TK
UniProt Number	P10721
UniProt Summary	FUNCTION: SwissProt: P10721 # This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K). SIZE: 976 amino acids; 109865 Da SUBUNIT: Interacts with APS. Interacts with the tenth PDZ domain of MPDZ. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DISEASE: SwissProt: P10721 # Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. & Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. & Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT). SIMILARITY: SwissProt: P10721 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Molecular Weight	76.7kDa.

Biological Information

Source

recombinant protein expressed in Sf21 cells

Specific Activity

169 U/mg, where one unit of cKit (V654A) activity is defined as 1nmol phosphate incorporated into 250 μM GGMEDIYFEFMGGKKK per minute at 30 °C with a final ATP concentration of 100 μM.

Entrez Gene Number

Entrez Gene Summary

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Symbol

KIT
C-Kit
PBT
CD117
SCFR
c-kit

Protein Target

c-Kit

Purification Method

Purified using glutathione-agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.

Target Sub-Family

UniProt Number

P10721

UniProt Summary

FUNCTION: SwissProt: P10721 # This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K).
SIZE: 976 amino acids; 109865 Da
SUBUNIT: Interacts with APS. Interacts with the tenth PDZ domain of MPDZ.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P10721 # Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. & Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. & Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT).
SIMILARITY: SwissProt: P10721 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.

Molecular Weight

76.7kDa.

Product Usage Statements
Quality Assurance	Routinely evaluated by phosphorylation of 250 μM GGMEDIYFEFMGGKKK. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active cKit (V654A).
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by phosphorylation of 250 μM GGMEDIYFEFMGGKKK.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active cKit (V654A).

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap. Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.

Storage and Shipping Information

Storage Conditions

Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.

Packaging Information
Material Size	10 µg

Packaging Information

Material Size

10 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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