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Anti-Hox A9 (rabbit polyclonal IgG)

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货号：07-178

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M EMSA, WB, ICC Rb Purified Polyclonal Antibody
Description
Catalogue Number 07-178
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Hox A9 Antibody
Background Information HOXA9 is a transcription factor with a central role in both haemopoiesis and leukaemia. High levels of HOXA9 expression in haemopoietic cells is a characteristic feature of acute myeloid leukaemia (AML), and may be sufficient to cause this disease. Overexpression of Hoxa-9 markedly expands hematopoietic stem cells. HOXA9 expression changes dramatically with age - a uniformly low level of expression during early adulthood is replaced by a frequently very high expression in adults over sixty.
References
Product Information
Format Purified
Control
  • Hoxa9 immortalized HF1 cells
Presentation Protein A purified immunoglobulin presented in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% Sodium Azide before the addition of glycerol to 30%.
Applications
Application Detect Hox A9 with Anti-Hox A9 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in EMSA, WB, ICC.
Key Applications
  • Electrophoretic Mobility Shift Assay
  • Western Blotting
  • Immunocytochemistry
Biological Information
Immunogen GST fusion protein corresponding to residues 194-272 of mouse Hox A9
Host Rabbit
Specificity Hox A9
Isotype IgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Based on a BLAST search, the antibody will likely cross react with Newt, Coelacanth, Dog, Chicken, and Rat Hox A9; Antelope, Mouse and Guinea Pig Hox 1.7; Frog, human and Zebrafish Hox A-9B; and Kenyan Clawed Frog Hox B.1
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is highly similar to the abdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and the NUP98 gene has been associated with myeloid leukemogenesis.
Gene Symbol
  • HOXA9
  • MGC1934
  • Hox-1G
  • HOX1.7
  • HOX1G
  • HOX1
  • ABD-B
Purification Method Protein A chromatography
UniProt Number
UniProt Summary FUNCTION: SwissProt: P31269 # Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
SIZE: 272 amino acids; 30172 Da
SUBCELLULAR LOCATION: Nucleus.
DISEASE: SwissProt: P31269 # A chromosomal aberration involving HOXA9 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with NUP98. & A chromosomal aberration involving HOXA9 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with MSI2.
SIMILARITY: SwissProt: P31269 ## Belongs to the Abd-B homeobox family. & Contains 1 homeobox DNA-binding domain.
Molecular Weight 36kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot on whole cell lysates of Hoxa9 immortalized HF1 myeloid progenitor cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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