Anti-Notch 4 (rabbit polyconal IgG)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Purified

Polyclonal Antibody

Description
Catalogue Number	07-189
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Notch 4 Antibody

Description

Catalogue Number

07-189

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Notch 4 Antibody

Product Information
Format	Purified
Presentation	0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide.

Product Information

Format

Purified

Presentation

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide.

Applications
Application	Anti-Notch 4 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Notch 4 & has been validated in WB.
Key Applications	Western Blotting

Applications

Application

Anti-Notch 4 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Notch 4 & has been validated in WB.

Key Applications

Western Blotting

Biological Information
Immunogen	His-tag fusion protein containing the carboxy-terminus of murine Notch 4
Host	Rabbit
Specificity	truncated, intracellular domain of Int3/Notch 4, possibly full-length Notch 4; does not cross-react with Notch 1, 2 or 3
Isotype	IgG
Species Reactivity	Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_004557
Entrez Gene Summary	This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. This gene may be associated with susceptibility to schizophrenia in a small portion of cases. An alternative splice variant has been described but its biological nature has not been determined.
Gene Symbol	NOTCH4 INT3 MGC74442 OTTHUMP00000062678 NOTCH3 hNotch4 notch4 FLJ16302
Purification Method	Protein A chromatography
UniProt Number	Q99466
UniProt Summary	FUNCTION: SwissProt: Q99466 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity). SIZE: 2003 amino acids; 209622 Da SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity). Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Notch 4 intracellular domain: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus. TISSUE SPECIFICITY: Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity). SIMILARITY: SwissProt: Q99466 ## Belongs to the NOTCH family. & Contains 5 ANK repeats. & Contains 28 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.
Molecular Weight	68kDa

Biological Information

Immunogen

His-tag fusion protein containing the carboxy-terminus of murine Notch 4

Host

Rabbit

Specificity

truncated, intracellular domain of Int3/Notch 4, possibly full-length Notch 4; does not cross-react with Notch 1, 2 or 3

Isotype

IgG

Species Reactivity

Mouse

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_004557

Entrez Gene Summary

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. This gene may be associated with susceptibility to schizophrenia in a small portion of cases. An alternative splice variant has been described but its biological nature has not been determined.

Gene Symbol

NOTCH4
INT3
MGC74442
OTTHUMP00000062678
NOTCH3
hNotch4
notch4
FLJ16302

Purification Method

Protein A chromatography

UniProt Number

Q99466

UniProt Summary

FUNCTION: SwissProt: Q99466 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity).
SIZE: 2003 amino acids; 209622 Da
SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity). Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Notch 4 intracellular domain: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
TISSUE SPECIFICITY: Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity).
SIMILARITY: SwissProt: Q99466 ## Belongs to the NOTCH family. & Contains 5 ANK repeats. & Contains 28 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.

Molecular Weight

68kDa

Product Usage Statements
Quality Assurance	routinely evaluated by immunoblot on lysates from 293 cells transfected with the intracellular domain of murine Notch 4
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

routinely evaluated by immunoblot on lysates from 293 cells transfected with the intracellular domain of murine Notch 4

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at 2-8°C for up to 12 months from date of receipt.

Storage and Shipping Information

Storage Conditions

Maintain at 2-8°C for up to 12 months from date of receipt.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Notch 4 (rabbit polyconal IgG)

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