Anti-CD8a (hu), PerCP-Cy5.5,clone RPA-T8

Species Reactivity	Key Applications	Host	Format	Antibody Type
Av, H, Ma, Yeast (S. cerevisiae)	IF, IP, WB, ICC, IHC	M	Ascites	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Av, H, Ma, Yeast (S. cerevisiae)

IF, IP, WB, ICC, IHC

Ascites

Monoclonal Antibody

Description
Catalogue Number	MAB1684
Replaces	04-590
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Caldesmon Antibody, clone TD107
Background Information	Caldesmon is a protein component of the thin filaments of smooth muscle myofibrils. It is also localized in the stress fibers of fibroblasts. Caldesmon was identified as a Ca2+/Calmodulin-binding protein with molecular weight of 120-150kDa (H-Caldesmon) and 70-80kDa (L-Caldesmon). H-Caldesmon is the primary isoform in smooth muscle while L-Caldesmon is most abundant in non-muscle cells. Caldesmon is capable of binding two calmodulin molecules, one at either end of the protein. Additionally, Caldesmon is an actin, myosin, and tropomyosin-binding protein. Human L-Caldesmon is a protein of 538 amino acids with mobility of 80kDa. In vitro, L-Caldesmon inhibits the actomyosin ATPase in an F-Actin-dependent manner. L-Caldesmon may play an important function in motile processes such as secretion and organelle movement.

Description

Catalogue Number

MAB1684

Replaces

04-590

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Caldesmon Antibody, clone TD107

Background Information

Caldesmon is a protein component of the thin filaments of smooth muscle myofibrils. It

is also localized in the stress fibers of fibroblasts. Caldesmon was identified as a

Ca2+/Calmodulin-binding protein with molecular weight of 120-150kDa (H-Caldesmon)

and 70-80kDa (L-Caldesmon). H-Caldesmon is the primary isoform in smooth muscle

while L-Caldesmon is most abundant in non-muscle cells. Caldesmon is capable of

binding two calmodulin molecules, one at either end of the protein. Additionally,

Caldesmon is an actin, myosin, and tropomyosin-binding protein. Human L-Caldesmon

is a protein of 538 amino acids with mobility of 80kDa. In vitro, L-Caldesmon inhibits

the actomyosin ATPase in an F-Actin-dependent manner. L-Caldesmon may play an

important function in motile processes such as secretion and organelle movement.

Product Information
Format	Ascites
Presentation	Ascites fluid. Liquid

Product Information

Format

Ascites

Presentation

Ascites fluid. Liquid

Applications
Application	Anti-Caldesmon Antibody, clone TD107 is an antibody against Caldesmon for use in IF, IP, WB, IC, IH.
Key Applications	Immunofluorescence Immunoprecipitation Western Blotting Immunocytochemistry Immunohistochemistry
Application Notes	Immunoblotting 1:50-1:100 Immunoprecipitation Immunofluorescence 3.7% formaldehyde fixation 10' 4C; permeabilization 0.2% triton X-100 in PBST for 5 minutes, blocked with 2% BSA in PBST for 20 minutes. Primary antibody 1:100 for 1 hour at room temperature {Garcia et al 2002}. Methanol fixation also is effective for clone TD107. Immunohistochemistry Optimal working dilutions must be determined by end user.

Applications

Application

Anti-Caldesmon Antibody, clone TD107 is an antibody against Caldesmon for use in IF, IP, WB, IC, IH.

Key Applications

Immunofluorescence
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry

Application Notes

Immunoblotting 1:50-1:100

Immunoprecipitation

Immunofluorescence

3.7% formaldehyde fixation 10' 4C; permeabilization 0.2% triton X-100 in PBST for 5 minutes, blocked with 2% BSA in PBST for 20 minutes. Primary antibody 1:100 for 1 hour at room temperature {Garcia et al 2002}.

Methanol fixation also is effective for clone TD107.

Immunohistochemistry

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Human platelet protein.
Clone	TD107
Host	Mouse
Specificity	Human caldesmon. Reacts with the caldesmon protein (80kD) (LMW form) from platelets by Western blotting.Crossreacts with the 140kD (HMW) isoform of smooth muscle. The HMW (120-150kDa) is predominantly expressed in smooth muscle whereas LMW caldesmon (70-80kDa) is found in nonmuscle tissue and cells. Neither of the two isoforms has been detected in skeletal muscle.
Isotype	IgG1
Species Reactivity	Avian Human Mammals Yeast (S. cerevisiae)
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_004342.5 NM_033138.2 NM_033139.2 NM_033140.2 NM_033157.2
Entrez Gene Summary	This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Gene Symbol	CALD1 Caldesmon CAD L-CAD CDM MGC21352 NAG22 H-CAD
UniProt Number	Q05682
UniProt Summary	FUNCTION: SwissProt: Q05682 # Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. SIZE: 793 amino acids; 93250 Da SUBCELLULAR LOCATION: Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) (By similarity). TISSUE SPECIFICITY: High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart. DOMAIN: SwissProt: Q05682 The N-terminal part seems to be a myosin/calmodulin- binding domain, and the C-terminal a tropomyosin/actin/calmodulin- binding domain. These two domains are separated by a central helical region in the smooth-muscle form. PTM: In non-muscle cells, phosphorylation by CDC2 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization (By similarity). SIMILARITY: Belongs to the caldesmon family.

Biological Information

Immunogen

Human platelet protein.

Clone

TD107

Host

Mouse

Specificity

Human caldesmon. Reacts with the caldesmon protein (80kD) (LMW form) from platelets by Western blotting.Crossreacts with the 140kD (HMW) isoform of smooth muscle.

The HMW (120-150kDa) is predominantly expressed in smooth muscle whereas LMW caldesmon (70-80kDa) is found in nonmuscle tissue and cells. Neither of the two isoforms has been detected in skeletal muscle.

Isotype

IgG1

Species Reactivity

Avian
Human
Mammals
Yeast (S. cerevisiae)

Antibody Type

Monoclonal Antibody

Entrez Gene Number

Entrez Gene Summary

This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Gene Symbol

CALD1
Caldesmon
CAD
L-CAD
CDM
MGC21352
NAG22
H-CAD

UniProt Number

Q05682

UniProt Summary

FUNCTION: SwissProt: Q05682 # Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
SIZE: 793 amino acids; 93250 Da
SUBCELLULAR LOCATION: Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) (By similarity).
TISSUE SPECIFICITY: High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
DOMAIN: SwissProt: Q05682 The N-terminal part seems to be a myosin/calmodulin- binding domain, and the C-terminal a tropomyosin/actin/calmodulin- binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
PTM: In non-muscle cells, phosphorylation by CDC2 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization (By similarity).
SIMILARITY: Belongs to the caldesmon family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain frozen at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain frozen at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-CD8a (hu), PerCP-Cy5.5,clone RPA-T8

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