Anti-CD8a (human), PE-Cy7, clone RPA-T8

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, Ca, H, M, Po, R, Rb	ELISA, FC, IP, WB, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

B, Ca, H, M, Po, R, Rb

ELISA, FC, IP, WB, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB1683
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Ankyrin Antibody, clone Ank016

Description

Catalogue Number

MAB1683

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Ankyrin Antibody, clone Ank016

Product Information
Format	Purified
Presentation	Purified immunoglobulin from culture supernatant. Liquid in 10 mM sodium phosphate, pH 7.5 and 150mM NaCl with 0.01% NaN3.

Product Information

Format

Purified

Presentation

Purified immunoglobulin from culture supernatant. Liquid in 10 mM sodium phosphate, pH 7.5 and 150mM NaCl with 0.01% NaN3.

Applications
Application	Anti-Ankyrin Antibody, clone Ank016 is an antibody against Ankyrin for use in ELISA, FC, IP, WB & IC.
Key Applications	ELISA Flow Cytometry Immunoprecipitation Western Blotting Immunocytochemistry
Application Notes	Western blotting at 1:200; low percentage PAGE gels a must; protein is quite large; proteinase inhibitors recommended or lower level fragments likely; membrane preparations and enhance signals. ELISA: 1:1000 Immunoprecipitation: 1:100 Immunocytochemistry, using 2% paraformaldehyde fixation; higher fixation (i.e. 4% PFA can be problematic) 1:100; light fixation 5-10' RT usually; permeabilize lightly with 0.1% triton X-100 as protein is intracellular. Flow cytometry: 1:500 Immunohistochemistry: 1:100; light fixations suggested; PLP or mild zinc formalin recommended. RIA: 1:5000 Optimal working dilutions must be determined by end user.

Applications

Application

Anti-Ankyrin Antibody, clone Ank016 is an antibody against Ankyrin for use in ELISA, FC, IP, WB & IC.

Key Applications

ELISA
Flow Cytometry
Immunoprecipitation
Western Blotting
Immunocytochemistry

Application Notes

Western blotting at 1:200; low percentage PAGE gels a must; protein is quite large; proteinase inhibitors recommended or lower level fragments likely; membrane preparations and enhance signals.

ELISA: 1:1000

Immunoprecipitation: 1:100

Immunocytochemistry, using 2% paraformaldehyde fixation; higher fixation (i.e. 4% PFA can be problematic) 1:100; light fixation 5-10' RT usually; permeabilize lightly with 0.1% triton X-100 as protein is intracellular.

Flow cytometry: 1:500

Immunohistochemistry: 1:100; light fixations suggested; PLP or mild zinc formalin recommended.

RIA: 1:5000

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Purified human erythroid cells ankyrin
Clone	Ank016
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Reacts with the intracellular ankyrin (216 kDa). Ankyrin consists of a single peptide of M.W. 215 kDa known to be involved in the attachment of spectrin containing membrane skeleton to the plasma membranes. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g., lymphocytes, platelets, fibroblasts and endothelial cells) and various tissues (e.g., brain, kidney, muscle and many epithelial tissues).
Isotype	IgG2a
Species Reactivity	Bovine Canine Human Mouse Pig Rat Rabbit
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_020479.2 NM_020481.2 NM_000037.3 NM_020475.2 NM_020476.2 NM_020477.2 NM_020478.3 NM_020480.3
Entrez Gene Summary	Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.
Gene Symbol	ANK1 ANK SPH2 SPH1 Ankyrin-R Ankyrin-1 ankyrin-R
UniProt Number	P16157
UniProt Summary	FUNCTION: SwissProt: P16157 # Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu17 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils. SIZE: 1881 amino acids; 206265 Da SUBUNIT: Interacts with a number of integral membrane proteins and cytoskeletal proteins. Binds SPTB/spectrin (beta chain) through a 70 AA N-terminal region of the 62 kDa domain, and the C-terminal of SLC4A1/erythrocyte membrane protein band 3 through the ankyrin repeat region. Also interacts with TTN/titin. Isoform Mu17 interacts with OBSCN isoform 3/obscurin. SUBCELLULAR LOCATION: Isoform Er1: Cytoplasm. Note=Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane. & Isoform Mu17: Membrane. Cytoplasm, myofibril, sarcomere, M-band. Note=Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells. & Isoform Mu18: Sarcoplasmic reticulum (Probable). & Isoform Mu19: Sarcoplasmic reticulum (Probable). & Isoform Mu20: Sarcoplasmic reticulum (Probable). TISSUE SPECIFICITY: Isoform Mu17, isoform Mu18,isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain. DOMAIN: SwissProt: P16157 The 55 kDa regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) and SLC4A1/erythrocyte membrane protein band 3. & The ANK repeat region forms a spiral around a large central cavity and is involved in binding of ion transporters. PTM: Regulated by phosphorylation. & Palmitoylated.DISEASE:SwissProt: P16157 # Defects in ANK1 are a cause of hereditary spherocytosis (HS) [MIM:182900]. Inheritance can be autosomal dominant or recessive. SIMILARITY: Contains 23 ANK repeats. & Contains 1 death domain. & Contains 1 ZU5 domain.

Biological Information

Immunogen

Purified human erythroid cells ankyrin

Clone

Ank016

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Reacts with the intracellular ankyrin (216 kDa). Ankyrin consists of a single peptide of M.W. 215 kDa known to be involved in the attachment of spectrin containing membrane skeleton to the plasma membranes. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g., lymphocytes, platelets, fibroblasts and endothelial cells) and various tissues (e.g., brain, kidney, muscle and many epithelial tissues).

Isotype

IgG2a

Species Reactivity

Bovine
Canine
Human
Mouse
Pig
Rat
Rabbit

Antibody Type

Monoclonal Antibody

Entrez Gene Number

Entrez Gene Summary

Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.

Gene Symbol

ANK1
ANK
SPH2
SPH1
Ankyrin-R
Ankyrin-1
ankyrin-R

UniProt Number

P16157

UniProt Summary

FUNCTION: SwissProt: P16157 # Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu17 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils.
SIZE: 1881 amino acids; 206265 Da
SUBUNIT: Interacts with a number of integral membrane proteins and cytoskeletal proteins. Binds SPTB/spectrin (beta chain) through a 70 AA N-terminal region of the 62 kDa domain, and the C-terminal of SLC4A1/erythrocyte membrane protein band 3 through the ankyrin repeat region. Also interacts with TTN/titin. Isoform Mu17 interacts with OBSCN isoform 3/obscurin.
SUBCELLULAR LOCATION: Isoform Er1: Cytoplasm. Note=Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane. & Isoform Mu17: Membrane. Cytoplasm, myofibril, sarcomere, M-band. Note=Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells. & Isoform Mu18: Sarcoplasmic reticulum (Probable). & Isoform Mu19: Sarcoplasmic reticulum (Probable). & Isoform Mu20: Sarcoplasmic reticulum (Probable).
TISSUE SPECIFICITY: Isoform Mu17, isoform Mu18,isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain.
DOMAIN: SwissProt: P16157 The 55 kDa regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) and SLC4A1/erythrocyte membrane protein band 3. & The ANK repeat region forms a spiral around a large central cavity and is involved in binding of ion transporters.
PTM: Regulated by phosphorylation. & Palmitoylated.DISEASE:SwissProt: P16157 # Defects in ANK1 are a cause of hereditary spherocytosis (HS) [MIM:182900]. Inheritance can be autosomal dominant or recessive.
SIMILARITY: Contains 23 ANK repeats. & Contains 1 death domain. & Contains 1 ZU5 domain.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-CD8a (human), PE-Cy7, clone RPA-T8

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