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Qiagen 凯杰 Biosharp Omega

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Anti-CD8a (human), APC-Cy7, clone RPA-T8

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号:MABF1669

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M FC, IP, WB R Purified Monoclonal Antibody
Description
Catalogue Number MAB1669
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-VEGF Receptor-2 Antibody, clone 89B3A5
Alternate Names
  • FLK-1
  • KDR
References
Product Information
Format Purified
Presentation Protein G purified immunoglobulin
Applications
Application This Anti-VEGF Receptor-2 Antibody, clone 89B3A5 is validated for use in FC, IP, WB for the detection of VEGF Receptor-2.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
Application Notes Immunoblotting

Immunoprecipitation

FACS Analysis Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Rat L cells transfected with the complete coding sequence of mouse FLK-1 cDNA.
Clone 89B3A5
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rat
Specificity FLK-1 is a receptor tyrosine kinase expressed on endothelial cells and on the common precursor of endothelial and hematopoietic stem cells Antibodies against FLK-1 are suitable for studies for endothelial development, for embryonal hematopoiesis, and for signal transduction via the FLK-1 receptor. It has not been tested whether the antibody blocks the binding of VEGF.
Isotype IgG2a
Species Reactivity
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Summary This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
Gene Symbol
  • VEGFR2
UniProt Number
UniProt Summary FUNCTION: SwissProt: P15692 # Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.
SIZE: 232 amino acids; 27042 Da
SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity).
SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon.
SIMILARITY: SwissProt: P15692 ## Belongs to the PDGF/VEGF growth factor family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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