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Anti-CD8a (hu), PerCP-Cy5.5, cl RPA-T8

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货号：MABF1685

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, Ca, H, M, Po, R, Rb ELISA, FC, IP, RIA, WB, ICC, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MAB1685
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Fodrin Antibody, clone FOD009
Alternate Names
  • Spectrin
References
Product Information
Format Purified
Presentation Liquid in 10 mM sodium phosphate, 150 mM NaCl, 0.01% NaN3 pH 7.5.
Applications
Application Anti-Fodrin Antibody, clone FOD009 detects level of Fodrin & has been published & validated for use in ELISA, FC, IP, RIA, WB, IC, IH.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Radioimmunoassay
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application Notes Western blotting at 1:200.

ELISA: 1:1000.

Immunoprecipitation: 1:100. Use rabbit capture secondary for best results.

Immunocytochemistry: light fixation with triton X-100 or acetone is recommended. 1:100.

Flow cytometry: 1:500 with permeabilization.

Immunohistochemistry: 1:100

RIA: 1:5000

Note: Catalog number MAB1622 is a more reactive and better antibody in most cases than MAB1685, particularly in non-human samples.

Optimal working dilutions must be determined by end user.

SUGGESTED WESTERN BLOT PROTOCOL FOR MAB1685:

1) Prepare protein samples by using appropriate lysis buffer e.g. RIPA (50 mM Tris HCl, 150 mM NaCl 5 mM EDTA, 1% Triton X-100, 100 mg/mL PMSF, 10 mg/mL leupeptin 20 mg/mL aprotinin).

2) Separate protein samples in SDS-PAGE 6% or 7.5%.

3) Blot onto the nitrocellulose membrane with TRANS-BLOT CELL (Bio-Rad) using constant current 100 mA overnight (Transfer buffer: 12.1 g Tris, 43.2 g glycine, 800 mL methanol to total volume of 4 liters).

4) Block membrane with 3% BSA in TBS (2.42 Tris, 8 g NaCl to total volume 1 liter, pH 7.6) 1-2 hours at room temperature.

5) Incubate membrane in diluted primary antibody solution (diluted in TTBS: 0.1% Tween-20 in TBS) one hour.

6) Wash membrane with TTBS: two quick rinses, 1 x 15 min, 3 x 5 min.

7) Incubate membrane with diluted HRP-conjugated secondary antibody solution (TTBS) for one hour.

8) Wash membrane with TTBS: 2 quick rinses, 1 x 15 min, 4 x 5 min.

9) Detect the signal with detection reagent following the instruction of manufacture.
Biological Information
Immunogen Purified bovine brain fodrin
Clone FOD009
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Reacts with the intracellular fodrin (240 kDa) in human, mouse, rabbit, rat and bovine cells/tissue. In non-erythroid cells, a spectrin-like protein has been identified as fodrin and found to contain two subunits (alpha subunit of M.W. 240 kDa and beta subunit of M.W. 235 kDa). Fodrin is believed to be involved in 1) stabilizing membrane structure; 2) maintaining cell shape; 3) linking the cytoskeleton to plasma membranes or intracellular vesicles. In particular, fodrin is known to mediate the association of actin filaments with the plasma membrane.
Isotype IgG2a
Species Reactivity
  • Bovine
  • Canine
  • Human
  • Mouse
  • Pig
  • Rat
  • Rabbit
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SPTAN1
  • FLJ44613
  • SPTA2
  • (ALPHA)II-SPECTRIN
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q13813 # Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
SIZE: 2472 amino acids; 284539 Da
SUBUNIT: Like erythrocyte spectrin, the spectrin-like proteins are capable of forming dimers which can further associate to tetramers. Interacts with isoform 1 of ACP1.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
PTM: Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro (By similarity).
SIMILARITY: SwissProt: Q13813 ## Belongs to the spectrin family. & Contains 3 EF-hand domains. & Contains 1 SH3 domain. & Contains 23 spectrin repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain frozen at -20°C for up to 12 months from date of receipt in undiluted aliquots. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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