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Qiagen 凯杰 Biosharp Omega

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Anti-CD45 (human), PE, clone HI30

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货号：MABF1598

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IH(P), IF Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number ABF210
Replaces MABF198
Description Anti-MDA5 Antibody
Alternate Names
  • MDA5 Antibody
  • Melanoma differentiation-associated protein 5
  • Interferon-induced with helicase C domain protein 1
  • IFIH1
Background Information Melanoma differentiation-associated protein 5 (MDA5), also known as Interferon-induced helicase C domain-containing protein 1 (IFIH1) is a member of the helicase family (RLR subfamily). MDA5 plays an important role in recognizing various viral components and triggers antiviral responses. When stimulated by dsRNA, MDA5 recruits adaptor protein VISA causing the activation of IRF-3 and NF-κB. MDA5 is widely expressed at low levels with barely detectable levels found in , testis and lung. Mutations in MDA5 have been associated with diabetes mellitus insulin-dependent type 19.
References
Product Information
Format Affinity Purified
Control
  • Daudi cell Lysate
Presentation Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Applications
Application Detect Melanoma differentiation-associated protein 5 using this rabbit polyclonal antibody, Anti-MDA5 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
Application Notes Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected MDA5 in human lymph node tissue lysate.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected MDA5 in human lymph node cells.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to the C-terminus of human MDA5.
Epitope C-terminus
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity This antibody recognizes the C-terminus of MDA5.
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Gene Symbol
  • IFIH1
Purification Method Antigen Affinity Purified
UniProt Number
Molecular Weight ~117 kDa observed. Uncharacterized bands may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected MDA5 in 15 µg of Daudi cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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