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Qiagen 凯杰 Biosharp Omega

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Anti-CD45 (human), APC-Cy7, clone HI30

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货号：MABF1591

品牌：Millipore 密理博

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Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IHC M Ascites Monoclonal Antibody
Description
Catalogue Number MABF191
Description Anti-CSF1 Antibody, clone 2D10
Alternate Names
  • Macrophage colony-stimulating factor 1
  • CSF-1
  • M-CSF
  • MCSF
  • Lanimostim
  • Processed macrophage colony-stimulating factor 1
Background Information Macrophage colony stimulating factor 1 (CSF1,CSF-1, M-CSF, MCSF) or Lanimostim is a cytokine that was first identified as a molecule that plays an important role in the survival, proliferation and differentiation of mononuclear cells such as phagocytes, macrophages and monocytes. Today however we know that CSF1 also serves several other functions in a variety of cell types. CSF1 is essential for normal bone development, osteoclast proliferation, and the resorption of bone. CSF1 is released by osteoclasts upon stimulation by parathyroid hormone. Additionally CSF1 impacts actin and cytoskeleton reorganization which effects cell adhesion and migration, and overstimulation of CSF1 in endothelial cells is linked to the development and progression of atherosclerosis. CSF1 is also highly expressed in the endometrial epithelium of the pregnant uterus and CSF1 is essential for normal embryonic implantation and placental development. CSF1 appears to play an important role in cancer as well; first CSF1 is an anti-tumor agent and increases macrophage expression of anti-tumor activity, but over expression of CSF1 in breast, bone, and several other forms of cancer appears to promote cell invasiveness, proliferation, and tumor survival. Finally, CSF1 as a secreted mature factor promotes the release of proinflammatory cytokines and serves as part of the innate immunity process for inflammation and infection. CSF1 is initially produced as a precursor that is membrane bound but processed and secreted upon stimulation. CSF1 is heavily glycosylated and found both in the ECM as well as surface membranes of cells. CSF1 receptor is found on numerous cell types including macrophages, endothelial cells, and osteoclasts. EMD-Millipore’s Anti-CSF1 mouse monoclonal antibody has been tested in western blot on recombinant protein and in paraffin embedded immunohistochemistry on human placental tissue sections.
References
Product Information
Format Ascites
Control
  • Human recombinant CSF1 protein
Presentation Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Applications
Application Anti-CSF1 Antibody, clone 2D10 is a highly specific mouse monoclonal antibody, that targets CSF1 & has been tested in western blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application Notes Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CSF1 in human placenta tissue.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Purified recombinant fragment of human CSF1 expressed in E. Coli.
Clone 2D10
Host Mouse
Isotype IgG1
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Gene Symbol
  • CSF1
Purification Method Unpurified
UniProt Number
Molecular Weight ~60 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in human recombinant CSF1 protein.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected human recombinant CSF1 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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