Anti-CD45 (human), PerCP, clone 2D1

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H, R	WB, IHC, FC, IP, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, H, R

WB, IHC, FC, IP, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	MABF106
Description	Anti-Viperin Antibody, clone MaP.VIP
Alternate Names	Radical S-adenosyl methionine domain-containing protein 2 Cytomegalovirus-induced gene 5 protein Viperin Virus inhibitory protein,endoplasmic reticulum-associated, interferon-inducible
Background Information	Virus inhibitory protein endoplasmic reticulum-associated interferon-inducible (Viperin) belongs to the RSAD2 family and is highly inducible by both type I and type II interferons, but little or no induction is observed in moncytic cell lines. Studies suggest that viperin is involved in host antiviral response, as viperin induction is observed from viral infections. Research also reveals that viperin inhibits influenza replication by disrupting its release from the plasmic membrane through the altering of plasma membrane fluidity by affecting formation of lipid rafts. Lipid rafts are known detergent-resistant microdomian sites for influenza budding.

Description

Catalogue Number

MABF106

Description

Anti-Viperin Antibody, clone MaP.VIP

Alternate Names

Radical S-adenosyl methionine domain-containing protein 2
Cytomegalovirus-induced gene 5 protein
Viperin
Virus inhibitory protein,endoplasmic reticulum-associated, interferon-inducible

Background Information

Virus inhibitory protein endoplasmic reticulum-associated interferon-inducible (Viperin) belongs to the RSAD2 family and is highly inducible by both type I and type II interferons, but little or no induction is observed in moncytic cell lines. Studies suggest that viperin is involved in host antiviral response, as viperin induction is observed from viral infections. Research also reveals that viperin inhibits influenza replication by disrupting its release from the plasmic membrane through the altering of plasma membrane fluidity by affecting formation of lipid rafts. Lipid rafts are known detergent-resistant microdomian sites for influenza budding.

Product Information
Format	Purified
Control	Mouse macrophage stimulated with IFN gamma and LPS
Presentation	Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

Mouse macrophage stimulated with IFN gamma and LPS

Presentation

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-Viperin Antibody, clone MaP.VIP detects level of Viperin & has been published & validated for use in WB, IH, FC, IP & IC.
Key Applications	Western Blotting Immunohistochemistry Flow Cytometry Immunoprecipitation Immunocytochemistry
Application Notes	Immunohistochemistry Analysis: A 1:500-1:1,000 dilution from a representative lot detected Viperin in human colon and rat liver tissues. Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HepG2 cell lysate (Hinson, E., et al. (2008). JBC. 284(7):4705–4712). Flow Cytometry Analysis: A representative lot was used by an independent laboratory in mouse liver, spleen, lung, and lymph node cells (Hinson, E., et al. (2010). J. Immunol. 184:5723-5731). Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in mouse spleen cells (Hinson, E., et al. (2010). J. Immunol. 184:5723-5731).

Applications

Application

Anti-Viperin Antibody, clone MaP.VIP detects level of Viperin & has been published & validated for use in WB, IH, FC, IP & IC.

Key Applications

Western Blotting
Immunohistochemistry
Flow Cytometry
Immunoprecipitation
Immunocytochemistry

Application Notes

Immunohistochemistry Analysis: A 1:500-1:1,000 dilution from a representative lot detected Viperin in human colon and rat liver tissues.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HepG2 cell lysate (Hinson, E., et al. (2008). JBC. 284(7):4705–4712).

Flow Cytometry Analysis: A representative lot was used by an independent laboratory in mouse liver, spleen, lung, and lymph node cells (Hinson, E., et al. (2010). J. Immunol. 184:5723-5731).

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in mouse spleen cells (Hinson, E., et al. (2010). J. Immunol. 184:5723-5731).

Biological Information
Immunogen	Recombinant protein corresponding to human Viperin.
Clone	MaP.VIP
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Isotype	IgG2aκ
Species Reactivity	Mouse Human Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_542388
Gene Symbol	RSAD2 CIG5
Purification Method	Protein G Purified
UniProt Number	Q8WXG1
UniProt Summary	FUNCTION: Involved in antiviral defense. May impair virus budding by disrupting lipid rafts at the plasma membrane, a feature which is essential for the budding process of many viruses. Acts through binding with and inactivating FPPS, an enzyme involved in synthesis of cholesterol, farnesylated and geranylated proteins, ubiquinones dolichol and heme. Plays a major role in the cell antiviral state induced by type I and type II interferon. Displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses, but not vesiculovirus. COFACTOR: Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (By similarity). SUBUNIT STRUCTURE: Interacts with FPPS. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus. Note: Probably associates with the cytosolic side of the endoplasmic reticulum. Infection with human cytomegalovirus (HCMV) causes relocation to the Golgi apparatus and to cytoplasmic vacuoles which also contain HCMV proteins glycoprotein B and pp28. INDUCTION: By interferon type I, type II and bacterial lipopolysaccharides (LPS). Little or no induction by IFNG/IFN-gamma is observed in monocytic cell lines. Induced by infection with human cytomegalovirus (HMCV), hepatitis C virus, yellow fever virus and Sendai virus, presumably through type I interferon pathway. MISCELLANEOUS: Up-regulated in atherosclerosis. Latent viruses like HCMV may be involved in atherogenesis by initiating local inflammation. This may induce up-regulation of antiviral gene RSAD2, which modulates lipids synthesis, and thus could play a role in abnormal lipid accumulation leading to atherosclerosis. SEQUENCE SIMILARITIES: Belongs to the RSAD2 family.
Molecular Weight	~42 kDa observed

Biological Information

Immunogen

Recombinant protein corresponding to human Viperin.

Clone

MaP.VIP

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Isotype

IgG2aκ

Species Reactivity

Mouse
Human
Rat

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_542388

Gene Symbol

RSAD2
CIG5

Purification Method

Protein G Purified

UniProt Number

Q8WXG1

UniProt Summary

FUNCTION: Involved in antiviral defense. May impair virus budding by disrupting lipid rafts at the plasma membrane, a feature which is essential for the budding process of many viruses. Acts through binding with and inactivating FPPS, an enzyme involved in synthesis of cholesterol, farnesylated and geranylated proteins, ubiquinones dolichol and heme. Plays a major role in the cell antiviral state induced by type I and type II interferon. Displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses, but not vesiculovirus. COFACTOR: Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (By similarity).

SUBUNIT STRUCTURE: Interacts with FPPS.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus. Note: Probably associates with the cytosolic side of the endoplasmic reticulum. Infection with human cytomegalovirus (HCMV) causes relocation to the Golgi apparatus and to cytoplasmic vacuoles which also contain HCMV proteins glycoprotein B and pp28.

INDUCTION: By interferon type I, type II and bacterial lipopolysaccharides (LPS). Little or no induction by IFNG/IFN-gamma is observed in monocytic cell lines. Induced by infection with human cytomegalovirus (HMCV), hepatitis C virus, yellow fever virus and Sendai virus, presumably through type I interferon pathway.

MISCELLANEOUS: Up-regulated in atherosclerosis. Latent viruses like HCMV may be involved in atherogenesis by initiating local inflammation. This may induce up-regulation of antiviral gene RSAD2, which modulates lipids synthesis, and thus could play a role in abnormal lipid accumulation leading to atherosclerosis.

SEQUENCE SIMILARITIES: Belongs to the RSAD2 family.

Molecular Weight

~42 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in mouse macrophage stimulated with IFN gamma and LPS. Western Blot Analysis: 1 µg/mL of this antibody detected Viperin in 10 µg of mouse macrophage stimulated with IFN gamma and LPS.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in mouse macrophage stimulated with IFN gamma and LPS.

Western Blot Analysis: 1 µg/mL of this antibody detected Viperin in 10 µg of mouse macrophage stimulated with IFN gamma and LPS.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-CD45 (human), PerCP, clone 2D1

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