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Qiagen 凯杰 Biosharp Omega

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Anti-CD45 (human), clone HI30

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号:MABF1609

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H FC, IHC, WB M PE-Cy7 Monoclonal Antibody
Description
Catalogue Number MABF609
Replaces 04-1102
Description Anti-CD45RA Antibody (human), PE-Cy7, clone HI100
Alternate Names
  • Receptor-type tyrosine-protein phosphatase C
  • Leukocyte common antigen
  • L-CA
  • T200
  • CD antigen CD45
Background Information The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others.The HI100 antibody is widely used as a marker for human CD45RA expression on naïve and activated T cells, B cells, and monocytes.
References
Product Information
Format PE-Cy7
Presentation Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Applications
Application This mouse monoclonal antibody Anti-CD45RA (human), PE-Cy7, clone HI100 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD45RA.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blotting
Biological Information
Immunogen Immunogen information for this antibody is proprietary information.
Clone HI100
Concentration 5 µL (0.25 µg)/test
Host Mouse
Isotype IgG2bκ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Gene Symbol
  • PTPRC
  • CD45
Purification Method Size Exclusion Chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD45RA in one million human peripheral blood lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size 25 tests
Transport Information
Supplemental Information
Specifications

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