Immobilon-P SQ 7x8.4cm sheet 50pk PVDF .2um

主要应用
西方蛋白印迹

主要应用

西方蛋白印迹

描述
产品目录编号	ISEQ07850
商名	Immobilon
描述	Immobilon-P^SQ 方片膜，PVDF，0.2 µm，7 x 8.4 cm
背景信息	Overview - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications. Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times. Immobilon-P Transfer Membrane: - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity Immobilon-E Membrane: - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa Blotting Sandwiches: - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device. Blotting Filter Paper: - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes. Immobilon-PSQ Membrane: - 0.2 µm pore size and a large internal structure - Higher protein adsorption and sequencing yields than other membranes - Recommended for blotting proteins less than 20 kDa - Prevents blow-through of low molecular weight proteins Immobilon-FL Membrane: - The first transfer membrane optimized for fluorescence applications - Extremely low background improves sensitivity of all fluorescence detection protocols - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths - Ideal for multiplexing and chemifluorescence applications Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications. Features & Benefits: - Won’t crack, curl or fracture when cut - Low background - Superior staining capabilities - Can be reprobed multiple times Applications: - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing; - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths

描述

产品目录编号

ISEQ07850

商名

Immobilon

描述

Immobilon-P^SQ 方片膜，PVDF，0.2 µm，7 x 8.4 cm

背景信息

Overview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths

产品信息
检测方法	呈色放射荧光
相容的染色剂	考马斯亮蓝酰胺黑印度墨水丽春红胶体金 CPTS 甲苯胺蓝透射显影
过滤器代码	ISEQ
过滤器颜色	白色

产品信息

检测方法

呈色放射荧光

相容的染色剂

考马斯亮蓝
酰胺黑
印度墨水
丽春红
胶体金
CPTS
甲苯胺蓝
透射显影

过滤器代码

ISEQ

过滤器颜色

白色

应用
应用	高得率的蛋白质测序、低分子量 Western blotting、氨基酸分析、N 端蛋白质定序和质谱分析
主要应用	西方蛋白印迹

应用

高得率的蛋白质测序、低分子量 Western blotting、氨基酸分析、N 端蛋白质定序和质谱分析

主要应用

西方蛋白印迹

生物信息
主结合机制	静电，疏水
BSA吸收性	340 µg/cm²
山羊IgG吸收性	448 µg/cm²
胰岛素吸收性	262 µg/cm²
可湿润性	疏水

生物信息

主结合机制

静电，疏水

BSA吸收性

340 µg/cm²

山羊IgG吸收性

448 µg/cm²

胰岛素吸收性

262 µg/cm²

可湿润性

疏水

理化信息
孔径	0.2 µm

理化信息

孔径

0.2 µm

尺寸
过滤器表面	平纹
过滤器尺寸	7 cm x 8.4 cm

尺寸

过滤器表面

平纹

过滤器尺寸

7 cm x 8.4 cm

材料信息
化学	疏水性聚偏氟乙烯（PVDF）

材料信息

化学

疏水性聚偏氟乙烯（PVDF）

包装信息
数量	50

包装信息

数量

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Immobilon-P SQ 7x8.4cm sheet 50pk PVDF .2um

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