Anti-PML Isoform V, clone 18B5.2 (mouse monoclonal)

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Anti-PML Isoform V, clone 18B5.2 (mouse monoclonal)

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货号：MABE172

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Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE172
Description	Anti-PML Isoform V Antibody, clone 18B5.2
Alternate Names	PML-5 PML-V Protein PML isoform V Promyelocytic leukemia protein isoform V RING finger protein 71 isoform V TRIM19beta Tripartite motif-containing protein 19 isoform V
Background Information	Protein PML (UniProt P29590; also known as Promyelocytic leukemia protein, RING finger protein 71, Tripartite motif-containing protein 19) is encoded by the PML (also known as MYL, PP8675, RNF71, TRIM19) gene (Gene ID 5371) in human. The human PML gene/protein adopted its name when it was originally discovered as an RARα fusion protein caused by chromosomal translocation in acute promyelocytic leukemia (APL) patients. In normal cells, the PML protein interacts with multiple protein partners in the nucleus to form PML nuclear bodies (PML-NBs) that mediate a variety of cellular functions including senescence, apoptosis, protein degradation, telemere stasbility, and antiviral defense. At least 12 PML isoforms are generated by alternative splicings, including six nuclear isoforms (PML-I to PML-VI) and the cytoplasmic isoform PML-VII (a.k.a. PML-VIIB), other spliced isoforms wihtout exon 6-coding sequence (a.a. 467-552) are likewise expected to be cytoplasmic due to the abscence of nuclear localization signal (NLS) encoded by exon 6. All isoforms share the same N-terminal region (a.a. 1-419) containing the RBCC/tripartite motif and may have related functions due to their common functional RBCC/TRIM domain, while increasing evidences also suggest that each PML isoform possesses distinct functions mediated by its specific C-terminal sequence. Isoform PML-1 (a.k.a. PML-I, TRIM19alpha; UniProt P29590-1) and PML-5 (a.k.a. PML-V or TRIM19beta; UniProt P29590-2) share the same N-terminal 570-a.a. sequence (encoded by exons 1 to 7a), however PML-1 derives its remaining C-terminal sequence (a.a. 571-882) from exons 8a & 9, while PML-V derives its (a.a. 571-611) from exon 7b with a different reading frame as that of PML-II.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-PML Isoform V Antibody, clone 18B5.2 is an antibody against PML Isoform V for use in Western Blotting, Immunocytochemistry.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Western Blotting Analysis: 10 µg/mL from a representative lot detected the expression of exogenously transfected human PML isoform V (PML-5), but not isoforms PML-1, -2, -3, -4, or -6, in lysates from transfected HEK293 cells (Courtesy of Professor Ygal Haupt, Peter MacCallum Cancer Centre, East Melbourne, Australia). Immunocytochemistry Analysis: 40 µg/mL from a representative lot immunostained 10% neutral-buffered formalin-fixed HEK293 cells transfected with human PML isoform V by non-fluorescent immunocytochemistry (Courtesy of Professor Ygal Haupt, Peter MacCallum Cancer Centre, East Melbourne, Australia). Immunocytochemistry Analysis: 10 µg/mL from a representative lot immunostained 4% paraformaldehyde-fixed HEK293 cells transfected with human PML isoform V by fluorescent immunocytochemistry (Courtesy of Professor Ygal Haupt, Peter MacCallum Cancer Centre, East Melbourne, Australia).

Biological Information
Immunogen	GST-tagged recombinant human PML-5 C-terminal fragment.
Epitope	C-terminal region.
Clone	18B5.2
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 18B5.2 reacts with PML spliced isoform V (PML-5), but not isoforms PML-1, -2, -3, -4, or -6.
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_150243
Gene Symbol	PML MYL PP8675 RNF71 TRIM19
Purification Method	Protein G Purified
UniProt Number	P29590-2
Molecular Weight	~75 kDa observed. 67.47 kDa calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

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Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in lysate from PML-5 transfected HEK293 cells. Western Blotting Analysis: 0.5 µg/mL of this antibody detected the exogenously expressed human PML-5 in 10 µg of lysate from transfected HEK293 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

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Anti-PML Isoform V, clone 18B5.2 (mouse monoclonal)

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