Anti-Nuclear Pore Complex Proteins, clone 414 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Yeast, R, M	ICC, IP, WB, EM	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, Yeast, R, M

ICC, IP, WB, EM

Purified

Monoclonal Antibody

Description
Catalogue Number	MABS1267
Description	Anti-Nuclear Pore Complex Proteins Antibody, clone 414
Alternate Names	Nuclear pore glycoprotein p62 62 kDa nucleoporin Nucleoporin Nup62 Nuclear pore complex proteins
Background Information	Nuclear pore glycoprotein p62 (UniProt P17955; also known as 62 kDa nucleoporin, Nucleoporin Nup62) is encoded by the Nup62 gene (Gene ID 65274) in rat species. The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. Soluble cargo-protein complexes navigate through the pore by binding to phenylalanine-glycine (FG)-repeat proteins attached to the channel walls. The Nup62 complex is located in the center of the NPC and contains the FG-repeat proteins Nup62, Nup54, and Nup58 associated with each other via conserved coiled-coil segments in a 1:1:1 stiochiometric ratio. The nuclear envelope breaks down during prometaphase. The disassembled nucleoporin subcomplexes act as critical regulators of various mitotic events to ensure proper chromosome segregation. Specifically, Nup62 is reported to localize to centrosomes/spindle poles, where it associates with the centrosomal proteins gamma-tubulin and hSAS-6. Nup62 is involved in the recruitment of critical proteins to the centrosome and its depletion results in mislocalization of several centrosomal components.

Description

Catalogue Number

MABS1267

Description

Anti-Nuclear Pore Complex Proteins Antibody, clone 414

Alternate Names

Nuclear pore glycoprotein p62
62 kDa nucleoporin
Nucleoporin Nup62
Nuclear pore complex proteins

Background Information

Nuclear pore glycoprotein p62 (UniProt P17955; also known as 62 kDa nucleoporin, Nucleoporin Nup62) is encoded by the Nup62 gene (Gene ID 65274) in rat species. The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. Soluble cargo-protein complexes navigate through the pore by binding to phenylalanine-glycine (FG)-repeat proteins attached to the channel walls. The Nup62 complex is located in the center of the NPC and contains the FG-repeat proteins Nup62, Nup54, and Nup58 associated with each other via conserved coiled-coil segments in a 1:1:1 stiochiometric ratio. The nuclear envelope breaks down during prometaphase. The disassembled nucleoporin subcomplexes act as critical regulators of various mitotic events to ensure proper chromosome segregation. Specifically, Nup62 is reported to localize to centrosomes/spindle poles, where it associates with the centrosomal proteins gamma-tubulin and hSAS-6. Nup62 is involved in the recruitment of critical proteins to the centrosome and its depletion results in mislocalization of several centrosomal components.

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Presentation

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-Nuclear Pore Complex Proteins Antibody, clone 414 is an antibody against Nuclear Pore Complex Proteins for use in Immunocytochemistry, Immunoprecipitation, Western Blotting, Electron Microscopy.
Key Applications	Immunocytochemistry Immunoprecipitation Western Blotting Electron Microscopy
Application Notes	Immunocytochemistry Analysis: A 1:200 dilution from a representative lot immunostained NIH/3T3 cell nuclear rim. Immunocytochemistry Analysis: A representative lot immunostained yeast nuclear envelope with a punctate and patchy pattern (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067). Immunocytochemistry Analysis: Representative lots detected a punctate staining pattern of Nup62 at the nuclear rim by fluorescent immunocytochemistry using 2% formaldehyde-fixed, methanol-permeabilized Buffalo rat liver (BRL) cells (Davis, L.I., and Blobel, G. (1987). Proc. Natl. Acad. Sci. U. S. A. 84(21):7552-755; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709). Electron Microscopy: A representative lot immunostained yeast nuclear envelope using 3% paraformaldehyde/0.2% glutaraldehyde-fixed yeast nuclei LR White sections (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067). Electron Microscopy: A representative lot immunostained the pore complexes in thin sections of isolated rat liver nuclei extracted with 2% Triton X-100 and fixed with 0.05% glutaraldehyde (Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709). Immunoprecipitation Analysis: A representative lot immunoprecipited a ~100 kDa (p110) and a ~95 kDa (p95) protein species from yeast nuclear extract. An additional ~55 kDa protein was immunoprecipitated by clone 414 using yeast cytosolic fraction or whole cell lysate (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067). Immunoprecipitation Analysis: A representative lot immunoprecipitated three major GlcNAcylated prtoein species of 62, 175, and 270 kDa from SDS-solubilized pore complex-lamina extract of Buffalo rat liver (BRL) cell nuclei preparation that had been labeled with UDP-[3H]Gal by galactosyltransferase (Davis, L.I., and Blobel, G. (1987). Proc. Natl. Acad. Sci. U. S. A. 84(21):7552-755). Immunoprecipitation Analysis: A representative lot immunoprecipitated GlcNAcylated 62 kDa protein (p62) from the SDS-solubilized pore complex-lamina extract, as well as a less glycosylated p61 cytoplasmic form from the postmitochondrial supernatant of Buffalo rat liver (BRL) cells (7552-755; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709). Western Blotting Analysis: A representative lot detected a ~100 kDa (p110) and a ~95 kDa (p95) immunoreactive bands in yeast nuclear extract (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067). Western Blotting Analysis: A representative lot detected a ~62 kDa (p62) and a ~200 kDa target bands associated with nuclear pore complex-lamina of rat liver nuclei preparation even following sequential nucleaases, Triton X-100, and 140 mM NaCl treatments (Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).

Applications

Application

Anti-Nuclear Pore Complex Proteins Antibody, clone 414 is an antibody against Nuclear Pore Complex Proteins for use in Immunocytochemistry, Immunoprecipitation, Western Blotting, Electron Microscopy.

Key Applications

Immunocytochemistry
Immunoprecipitation
Western Blotting
Electron Microscopy

Application Notes

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot immunostained NIH/3T3 cell nuclear rim.
Immunocytochemistry Analysis: A representative lot immunostained yeast nuclear envelope with a punctate and patchy pattern (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067).
Immunocytochemistry Analysis: Representative lots detected a punctate staining pattern of Nup62 at the nuclear rim by fluorescent immunocytochemistry using 2% formaldehyde-fixed, methanol-permeabilized Buffalo rat liver (BRL) cells (Davis, L.I., and Blobel, G. (1987). Proc. Natl. Acad. Sci. U. S. A. 84(21):7552-755; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).
Electron Microscopy: A representative lot immunostained yeast nuclear envelope using 3% paraformaldehyde/0.2% glutaraldehyde-fixed yeast nuclei LR White sections (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067).
Electron Microscopy: A representative lot immunostained the pore complexes in thin sections of isolated rat liver nuclei extracted with 2% Triton X-100 and fixed with 0.05% glutaraldehyde (Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).
Immunoprecipitation Analysis: A representative lot immunoprecipited a ~100 kDa (p110) and a ~95 kDa (p95) protein species from yeast nuclear extract. An additional ~55 kDa protein was immunoprecipitated by clone 414 using yeast cytosolic fraction or whole cell lysate (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067).
Immunoprecipitation Analysis: A representative lot immunoprecipitated three major GlcNAcylated prtoein species of 62, 175, and 270 kDa from SDS-solubilized pore complex-lamina extract of Buffalo rat liver (BRL) cell nuclei preparation that had been labeled with UDP-[3H]Gal by galactosyltransferase (Davis, L.I., and Blobel, G. (1987). Proc. Natl. Acad. Sci. U. S. A. 84(21):7552-755).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GlcNAcylated 62 kDa protein (p62) from the SDS-solubilized pore complex-lamina extract, as well as a less glycosylated p61 cytoplasmic form from the postmitochondrial supernatant of Buffalo rat liver (BRL) cells (7552-755; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).
Western Blotting Analysis: A representative lot detected a ~100 kDa (p110) and a ~95 kDa (p95) immunoreactive bands in yeast nuclear extract (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067).
Western Blotting Analysis: A representative lot detected a ~62 kDa (p62) and a ~200 kDa target bands associated with nuclear pore complex-lamina of rat liver nuclei preparation even following sequential nucleaases, Triton X-100, and 140 mM NaCl treatments (Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).

Biological Information
Immunogen	Triton X-100 treated rat liver nuclei.
Clone	414
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 414 immunostained rat liver cell nuclear rim and yeast cell nuclear envelope (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).
Isotype	IgG1κ
Species Reactivity	Human Yeast Rat Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_075586
Gene Symbol	Nup62
Purification Method	Protein G Purified
UniProt Number	P17955
Molecular Weight	53.40 kDa (Rat Nup62) calculated. ~62/175/270 kDa (Rat liver nuclear extract) and ~95/100 kDa (Yeast nuclear extract) reported (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709). Uncharacterized band(s) may appear in some lysates.

Biological Information

Immunogen

Triton X-100 treated rat liver nuclei.

Clone

414

Concentration

Please refer to lot specific datasheet.

Host

Mouse

Specificity

Clone 414 immunostained rat liver cell nuclear rim and yeast cell nuclear envelope (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).

Isotype

IgG1κ

Species Reactivity

Human
Yeast
Rat
Mouse

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_075586

Gene Symbol

Nup62

Purification Method

Protein G Purified

UniProt Number

P17955

Molecular Weight

53.40 kDa (Rat Nup62) calculated. ~62/175/270 kDa (Rat liver nuclear extract) and ~95/100 kDa (Yeast nuclear extract) reported (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709). Uncharacterized band(s) may appear in some lysates.

Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:200 dilution of this antibody immunostained HeLa cell nuclear rim.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:200 dilution of this antibody immunostained HeLa cell nuclear rim.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Packaging Information

Material Size

100 μg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Nuclear Pore Complex Proteins, clone 414 (mouse monoclonal)

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