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Anti-TBX1, clone 7E12.1 (mouse monoclonal)

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货号：MABN720

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB M Culture Supernatant Monoclonal Antibody
Description
Catalogue Number MABN720
Replaces AB15624
Description Anti-TBX1 Antibody, clone 7E12.1
Alternate Names
  • T-box transcription factor TBX1
  • T-box protein 1
  • Testis-specific T-box protein
Background Information T-box transcription factor TBX1 (UniProt O43435; also known as T-box protein 1, Testis-specific T-box protein) is encoded by the TBX1 (also known as CTHM, DGS, TBX1C, VCFS) gene (Gene ID 6899) in human. TBX1 blongs to the T-box family of transcription factors that play important roles in the formation of tissues and organs during embryonic development. TBX1 is required for the second heart field (SHF) development for outflow tract development by regulating proliferation and differentiation of SHF cells. TBX1 is also shown to negatively regulate thymic epithelial cell (TEC) growth and differentiation in mice, extinction of Tbx1 gene expression in the third pharyngeal pouch (3rd pp) endoderm is a prerequisite for thymus organogenesis. Human TBX mutations cause DiGeorge syndrome, characterized by parathyroid hypoplasia, thymic hypoplasia, defects in the outflow tract of the heart, and craniofacial anomalies.
References
Product Information
Format Culture Supernatant
Presentation Mouse monoclonal IgG2aκ hybridoma culture supernatant without preservatives.
Applications
Application Anti-TBX1 Antibody, clone 7E12.1 is an antibody against TBX1 for use in Western Blotting.
Key Applications
  • Western Blotting
Application Notes Western Blotting Analysis: A 1:500 dilution from a representative lot detected TBX1 in 10 µg of mouse Neuro-2a and human SH-SY5Y neuroblastoma cell lysates.
Biological Information
Immunogen GST-tagged recombinant human TBX1 C-terminal fragment.
Epitope C-terminal region.
Clone 7E12.1
Concentration Please refer to lot specific datasheet.
Host Mouse
Specificity Clone 7E12.1 was raised against a recombinant fragment derived from TBX1 spliced isoform A (Uniprot O43435-1), cross-reactivity toward spliced isoform B and isoform C (TBX1C) is possible, but has not been determined.
Isotype IgG2aκ
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TBX1
  • CTHM
  • DGS
  • TBX1C
  • VCFS
Purification Method Unpurified
UniProt Number
Molecular Weight ~48 kDa observed. 43.13 kDa (isoform A), 40.35 kDa (isoform B), and 52.67 kDa (isoform C; TBX1C) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TBX1 in 10 µg of HUVEC lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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