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Rb X DEDAF

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0.00
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货号：AB3637

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Rb Purified Polyclonal Antibody
Description
Catalogue Number AB3637
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-DEDAF Antibody
References
Product Information
Format Purified
Presentation Purified IgG by immunoaffinity chromatography. Liquid in PBS containing 0.02% sodium azide.
Applications
Application This Anti-DEDAF Antibody is validated for use in WB for the detection of DEDAF.
Key Applications
  • Western Blotting
Application Notes Western blot: 0.5-1 μg/mL.

A549 or HepG2 cell lysate can be used as a positive control and a 32 kDa band should be detected.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Rabbit anti-DEDAF polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 215 to 228 of human-DEDAF. This sequence is identical to the mouse sequence.
Host Rabbit
Specificity Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. DEDAF (DED Associated Factor) is expressed in multiple tissues and cell lines. DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/Caspase-8 complexes at the cell membrane. DEDAF also interacts with DEDD (DED-containing DNA binding) protein in the nucleus, which indicates its involvement in the regulation of both cytoplasmic and nuclear events of apoptosis.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RYBP
  • DEDAF
  • YEAF1
  • AAP1
  • APAP-1
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q8N488 # May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. In tumor cell lines, may induce apoptosis.
SIZE: 228 amino acids; 24822 Da
SUBUNIT: Interacts with CBX2, YAF2, RING1 and RNF2 (By similarity). Interacts with apoptin, DEDD, FADD, CASP8, CASP10, YY1 and E4TF1B. Together with E4TF1B and YY1, it forms a ternary complex, probably being the bridge factor between these two transcription factors.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Primarily found in the nucleus.
TISSUE SPECIFICITY: Widely expressed with highest levels in lymphoid tissues and placenta.
SIMILARITY: SwissProt: Q8N488 ## Contains 1 RanBP2-type zinc finger.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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