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Rb X Endonuclease G

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货号：AB3639

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB3639
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Endonuclease G Antibody
Alternate Names
  • EndoG
References
Product Information
Format Affinity Purified
Presentation Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
Applications
Application Anti-Endonuclease G Antibody is an antibody against Endonuclease G for use in WB.
Key Applications
  • Western Blotting
Application Notes Western blotting (1-2μg/mL)

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide corresponding to amino acids 55 to 70 of human EndoG (GGPRGPGELAKYGLP).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Endonuclease G (EndoG), a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. EndoG is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2).
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
Gene Symbol
  • ENDOG
  • FLJ27463
  • EC 3.1.30.-
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q14249 # Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity).
COFACTOR: Manganese or magnesium (By similarity).
SIZE: 297 amino acids; 32646 Da
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: SwissProt: Q14249 ## Belongs to the DNA/RNA non-specific endonuclease family.
MISCELLANEOUS: The active site contains 1 hydrated divalent metal cation that has only 1 direct interaction with the protein; all other interactions are via water molecules (By similarity).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8ºC for 12 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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