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Rb X Dopamine beta Hydroxylase N-Terminal

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货号：AB1538

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, H, R, Sh IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB1538
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Dopamine β Hydroxylase Antibody, NT
Alternate Names
  • DBH
References
Product Information
Format Affinity Purified
Presentation Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Applications
Application Detect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase Antibody, N-terminus validated for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Immunoblotting: 1:250-1:1,000.

Immunohistochemistry: 1:250-1:1,000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain Showed weak staining on both human and monkey.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide, CSAPRESPLPYHIPLDPEG-amide, cloned from human (DbetaH) cDNA (Nagatsu et al. 1990).
Epitope N-terminus
Host Rabbit
Specificity Dopamine Beta Hydroxylase (DbetaH) N-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. »70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE.
Species Reactivity
  • Bovine
  • Human
  • Rat
  • Sheep
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.
Gene Symbol
  • DBH
  • DBM
  • EC 1.14.17.1 [Contains: Soluble dopamine beta-hydroxylase].
UniProt Number
UniProt Summary FUNCTION: SwissProt: P09172 # Conversion of dopamine to noradrenaline.
COFACTOR: Binds 1 PQQ per subunit. & Binds 2 copper ions per subunit.
SIZE: 617 amino acids; 69065 Da
SUBUNIT: Homotetramer composed of two non-covalently bound disulfide-linked dimers.
SUBCELLULAR LOCATION: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. & Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single- pass type II membrane protein (Potential).
DISEASE: SwissProt: P09172 # Defects in DBH are the cause of DBH deficiency [MIM:223360]; also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
SIMILARITY: SwissProt: P09172 ## Belongs to the copper type II ascorbate-dependent monooxygenase family. & Contains 1 DOMON domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze-thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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