Anti-Nerve Growth Factor Receptor, p75

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	ICC, IH(P), IP	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

ICC, IH(P), IP

Serum

Polyclonal Antibody

Description
Catalogue Number	AB1554
Replaces	04-1111
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Nerve Growth Factor Receptor Antibody, p75
Alternate Names	CD271 antigen Low affinity neurotrophin receptor p75NTR Low-affinity nerve growth factor receptor NGF receptor low affinity nerve growth factor receptor nerve growth factor receptor nerve growth factor receptor (TNFR superfamily, member 16) p75 ICD
Background Information	Nerve Growth Factor Receptor p75 (UniProt: Q9Z0W1; also known as Tumor necrosis factor receptor superfamily member 16, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, CD271) is encoded by the Ngfr (also known as Tnfrsf16) gene in murine species. Nerve Growth Factor Receptor p75 is a single-pass type I, homodimeric membrane protein that can serve as a low affinity receptor for NGF, BDNF, NT-3, and NT-4. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 22-246), a transmembrane domain (aa 247-265), and a cytoplasmic domain (aa 266-417). Its expression is shown to oscillate in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain and in liver and expression is seen at higher levels during the light period and lower during dark period. Depending on the developmental stage, cell type, and whether they are unbound or ligand bound, and the type of ligand, p75 can activate apoptosis or survival. During development, it signals toward apoptosis to eliminate unnecessary neurons. Its death domain is localized to amino acids 346-411, which is responsible for its interaction with RAN-binding protein 9 (RANBP9). Neurotrophin binding to p75 can activate the MAP kinase pathway leading to cell survival or apoptosis depending on the cell type or ligand type.

Description

Catalogue Number

AB1554

Replaces

04-1111

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Nerve Growth Factor Receptor Antibody, p75

Alternate Names

CD271 antigen
Low affinity neurotrophin receptor p75NTR
Low-affinity nerve growth factor receptor
NGF receptor
low affinity nerve growth factor receptor
nerve growth factor receptor
nerve growth factor receptor (TNFR superfamily, member 16)
p75 ICD

Background Information

Nerve Growth Factor Receptor p75 (UniProt: Q9Z0W1; also known as Tumor necrosis factor receptor superfamily member 16, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, CD271) is encoded by the Ngfr (also known as Tnfrsf16) gene in murine species. Nerve Growth Factor Receptor p75 is a single-pass type I, homodimeric membrane protein that can serve as a low affinity receptor for NGF, BDNF, NT-3, and NT-4. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 22-246), a transmembrane domain (aa 247-265), and a cytoplasmic domain (aa 266-417). Its expression is shown to oscillate in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain and in liver and expression is seen at higher levels during the light period and lower during dark period. Depending on the developmental stage, cell type, and whether they are unbound or ligand bound, and the type of ligand, p75 can activate apoptosis or survival. During development, it signals toward apoptosis to eliminate unnecessary neurons. Its death domain is localized to amino acids 346-411, which is responsible for its interaction with RAN-binding protein 9 (RANBP9). Neurotrophin binding to p75 can activate the MAP kinase pathway leading to cell survival or apoptosis depending on the cell type or ligand type.

Product Information
Format	Serum
Control	Rat PC12 cells.
Presentation	Rabbit polyclonal serum containing 0.05% NaN3.

Product Information

Format

Serum

Control

Rat PC12 cells.

Presentation

Rabbit polyclonal serum containing 0.05% NaN3.

Applications
Application	Anti-Nerve Growth Factor Receptor Antibody, p75 is an antibody against Nerve Growth Factor Receptor for use in IC, IH(P),WB and IP.
Key Applications	Immunocytochemistry Immunohistochemistry (Paraffin) Immunoprecipitation
Application Notes	Immunoprecipitation: 1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate. Immunohistochemistry(paraffin): 1:50 and 1:300 dilution from a previous lot detected NGF Receptor p75 using IHC-Select Detection with HRP-DAB. Immunocytochemistry: 1:500 dilution from a previous lot detected NGF Receptor p75 in rat PC12 cells.

Applications

Application

Anti-Nerve Growth Factor Receptor Antibody, p75 is an antibody against Nerve Growth Factor Receptor for use in IC, IH(P),WB and IP.

Key Applications

Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation

Application Notes

Immunoprecipitation:
1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.

Immunohistochemistry(paraffin):
1:50 and 1:300 dilution from a previous lot detected NGF Receptor p75 using IHC-Select Detection with HRP-DAB.

Immunocytochemistry:
1:500 dilution from a previous lot detected NGF Receptor p75 in rat PC12 cells.

Biological Information
Immunogen	Recombinant mouse NGF Receptor p75 extracellular fragment.
Epitope	extracellular
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	p75 nerve growth factor receptor (p75 Neurotrophin receptor, low affinity neurotrophin receptor), extracellular domain.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	It has been reported that this antibody does not recognize rat p75 by Western blot. See MAB365 for rat reactive antibody using Western blotting.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_150086.2
Entrez Gene Summary	Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
Gene Symbol	NGFR p75(NTR) TNFRSF16 Gp80-LNGFR CD271
Purification Method	Unpurified
UniProt Number	Q9Z0W1
UniProt Summary	FUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SIZE: 427 amino acids; 45183 Da SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.
Molecular Weight	44.7 kDa

Biological Information

Immunogen

Recombinant mouse NGF Receptor p75 extracellular fragment.

Epitope

extracellular

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

p75 nerve growth factor receptor (p75 Neurotrophin receptor, low affinity neurotrophin receptor), extracellular domain.

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

It has been reported that this antibody does not recognize rat p75 by Western blot. See MAB365 for rat reactive antibody using Western blotting.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_150086.2

Entrez Gene Summary

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Gene Symbol

NGFR
p75(NTR)
TNFRSF16
Gp80-LNGFR
CD271

Purification Method

Unpurified

UniProt Number

Q9Z0W1

UniProt Summary

FUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
SIZE: 427 amino acids; 45183 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9.
PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues.
SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.

Molecular Weight

44.7 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by immunoprecipitation on PC12 cell lysates. Immunoprecipitation (IP): 1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by immunoprecipitation on PC12 cell lysates.

Immunoprecipitation (IP): 1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-Nerve Growth Factor Receptor, p75

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