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Rb X Dopamine Receptor D1A

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号:AB1765P

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Po, R ELISA, IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB1765P
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Dopamine D1A Receptor Antibody, CT, cytoplasmic
References
Product Information
Format Affinity Purified
Presentation Affinity purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% BSA.
Applications
Application Anti-Dopamine D1A Receptor Antibody, C-terminus, cytoplasmic is an antibody against Dopamine D1A Receptor for use in ELISA, IH & WB.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang et al. 1992; O'Connel et al. 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang et al. 1992; O'Connel et al. 1995) that is present in relatively small amounts.

Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 μg/mL (1 μg/mL D1A peptide (Cat. Number AG259)/well)

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen A 13 amino acid peptide sequence (amino acids 403-415) from rat D1A (Monsma et al. 1990; Zhou et al. 1990; Zhou et al. 1992; O'Dowd et al. 1990). This peptide is predicted to be cytoplasmic.
Epitope C-terminus, cytoplasmic
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Dopamine Receptor D1A. An antibody made to the D1R peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes by Western blot. The 75 kDa band was not visible in striatum (Huang et al. 1992). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band. By immunohistochemistry D1R has been localized in various regions of the rat brain (Huang et al. 1992).

May recognize porcine or bovine (100 % similarity) or human (78% similarity).
Species Reactivity
  • Pig
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
Gene Symbol
  • DRD1
  • DRD1A
  • DADR
  • D
UniProt Number
UniProt Summary FUNCTION: SwissProt: P21728 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 446 amino acids; 49293 Da
SUBUNIT: Interacts with DNAJC14 via its C-terminus (By similarity). Interacts with DRD1IP.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14 (By similarity).
TISSUE SPECIFICITY: Detected in caudate, nucleus accumbens and in the olfactory tubercle.
SIMILARITY: SwissProt: P21728 ## Belongs to the G-protein coupled receptor 1 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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