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Rabbit Anti-Dopamine D1A Receptor

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货号：AB1784P

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R ELISA, IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB1784P
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain
References
Product Information
Format Affinity Purified
Control
  • POSITIVE CONTROL: rat striatal membranes (western blot)
Presentation Affinity purified immunoglobulin. Liquid in PBS with 0.2% BSA and 0.05% sodium azide.
Applications
Application Detect Dopamine D1A Receptor using this Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain validated for use in ELISA, IH & WB.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Western Blotting
Application Notes Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed tissue sections. D1R has been localized in various regions of the rat brain (Huang, 1992; O'Connel, 1995)

Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang, 1992; O'Connel, 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang, 1992; O'Connel, 1995) that is present in relatively small amounts.

ELISA: 0.5-1.0 μg/mL using 1 μg/mL of control peptide per well.

The immunogen peptide is available (Cat. No. AG266) for use in ELISA or antibody blocking studies. AG266 is not recommended for use as a western blot positive control.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen A nine amino acid peptide corresponding to the 4th extracellular domain of the rat D1A receptor. The immunogen is absolutely conserved in the mouse D1A receptor and shows 88% homology with human and 77% with porcine and bovine D1A receptors. The peptide shows no significant sequence similarity with other dopamine receptors (D1B, D2-D5).
Epitope 4th Extracellular Domain
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Dopamine D1A Receptor (D1R). The rat D1A receptor is a 446 amino acid, G-protein coupled transmembrane receptor protein (Monsma, 1990; Zhou, 1990; Zhou, 1992; O'Dowd, 1990). The immunogen sequence has 88% sequence similarity with human and 77% with porcine.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
Gene Symbol
  • DRD1
  • DRD1A
  • DADR
  • D
UniProt Number
UniProt Summary FUNCTION: SwissProt: P21728 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 446 amino acids; 49293 Da
SUBUNIT: Interacts with DNAJC14 via its C-terminus (By similarity). Interacts with DRD1IP.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14 (By similarity).
TISSUE SPECIFICITY: Detected in caudate, nucleus accumbens and in the olfactory tubercle.
SIMILARITY: SwissProt: P21728 ## Belongs to the G-protein coupled receptor 1 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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