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Anti-MELK, clone 6C1.3 (mouse monoclonal)

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货号：MAB4331

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB M Purified Monoclonal Antibody
Description
Catalogue Number MAB4331
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-MELK Antibody, clone 6C1.3
Alternate Names
  • Maternal Embryonic Leucine Zipper Kinase
Background Information Melk, (Maternal Embryonic Leucine Zipper Kinase) is a serine-threonine kinase of the snfI/AMPK family that is expressed in developing neuroepithelium, self-renewing neural stem cells and granule cell precursors. It is also considered an important brain tumor marker, being highly expressed in gliobastoma, astrocytomas, and medulablastomas.
References
Product Information
Format Purified
Presentation Purified ascites. Liquid in PBS, pH 7.0. Contains 0.1% sodium azide.
Applications
Application This Anti-MELK Antibody, clone 6C1.3 is validated for use in WB for the detection of MELK.
Key Applications
  • Western Blotting
Application Notes Western blot: Recommended dilution: 1:500 (2 μg/mL)
Expected Molecular Weight: 74.5 kDa
Note: Bands may be present at a higher molecular weight due to glycosolation.
Biological Information
Immunogen MELK Recombinant protein.
Clone 6C1.3
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG2κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MELK
  • hMELK
  • HPK38
  • KIAA0175
  • hPK38
  • OTTHUMP00000046113
  • EC 2.7.11.1
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q14680 # Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
SIZE: 651 amino acids; 74642 Da
SUBUNIT: Interacts with ZNF622 and PPP1R8.
SUBCELLULAR LOCATION: Cytoplasm (Potential).
TISSUE SPECIFICITY: Expressed in placenta, kidney, thymus, testis, ovary and intestine.
PTM: Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8 (Probable).
SIMILARITY: SwissProt: Q14680 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 KA1 (kinase-associated) domain. & Contains 1 protein kinase domain.
Stem Cell Type
  • Neural Stem Cells
Molecular Weight 74.5 kDA; glycosylations may result in higher MW bands in western blot.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 1 year at 2-8 °C from date of shipment
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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