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Rb X Adenosine A1 Receptor

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货号：AB1587P

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Gp, H, Po, R, Sh ELISA, ICC, IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB1587P
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Adenosine A1 Receptor Antibody
References
Product Information
Format Affinity Purified
Control
  • Brain tissue
Presentation Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
Applications
Application Anti-Adenosine A1 Receptor Antibody detects level of Adenosine A1 Receptor & has been published & validated for use in ELISA, IC, IH & WB.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application Notes Immunohistochemistry: 10-50 μg/mL on frozen tissue sections. Light fixation is recommended. 2% 0.2% picric acid {Cooke, HJ (1999) AJP Gastrointest Liver Physiol 276:4516-4626}

Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.

Western blot: 1-10 μg/mL (Chemiluminescence technique): Membrane preparations are recommended for enhanced signals {Smith et al (2001) Biochem Pharmacology 61:237-244} Higher concentrations of the antibody may be necessary if calorimetric detection or milk-based antibody diluent are used.

ELISA: 1:10,000-1:50,000 (50-100 ng A1R peptide (Cat. Number AG289)/well)

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Amino acid sequence corresponding to the 3rd extracellular domain (amino acids 163-176, GEPVIKCEFEKVIS) of the rat A1R gene.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Adenosine A1 receptor (A1R). Recognizes the 79 kDa and 39 kDa bands of in cortical membranes from rat, porcine and lamb. The 79 kDa band can be converted to 39 kDa band by agonist or antagonist treatment and could represent a A1R dimers.
Species Reactivity
  • Guinea Pig
  • Human
  • Pig
  • Rat
  • Sheep
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene.
Gene Symbol
  • ADORA1
  • RDC7
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P30542 # Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
SIZE: 326 amino acids; 36512 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P30542 ## Belongs to the G-protein coupled receptor 1 family.
Molecular Weight 79 kDa and 39 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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