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Rbt X Hu LH

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货号：AB944

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Ca, H, R, Sh, Fe, Ox IH(P) Rb Serum Polyclonal Antibody
Description
Catalogue Number AB944
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Luteinizing Hormone Antibody
Alternate Names
  • LH
References
Product Information
Format Serum
Presentation Liquid rabbit serum containing 0.1% sodium azide.
Applications
Application This Anti-Luteinizing Hormone Antibody is validated for use in IH(P) for the detection of Luteinizing Hormone.
Key Applications
  • Immunohistochemistry (Paraffin)
Application Notes Immunofluorescence and immunoperoxidase staining of formalin-fixed and paraffin-embedded sections of anterior pituitary glands. Cryostat sections of the same tissues can also be used.

Optimal working dilutions must be determined by the end user.

IMMUNOPEROXIDASE STAINING:

1. Hydrate the sections with PBS, pH 7.4.

2. Incubate the section with antiserum diluted 1:1,000 in PBS at 37°C for 30 minutes.

3. Wash for 15 minutes with PBS at room temperature and treat sequentially with Goat anti-Rabbit IgG and PAP complex at 37°C. Wash excess reagent from the section as described above.

4. Treat the section with a mixture of 3,3'-diaminobenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections may be dehydrated, cleared and mounted.

IMMUNOFLUORESCENCE STAINING:

1. Same as Step 1 above.

2. Incubate the section with antiserum diluted 1:20-1:40 in PBS at 37°C for 30 minutes.

3. After washing off the excess antiserum with PBS, treat the section with fluorescein labeled Goat anti-Rabbit IgG for 30 minutes at 37°C. Wash the tissue with PBS and mount under glycerine-saline.
Biological Information
Immunogen ANTIGEN:

Highly purified LH
Host Rabbit
Specificity Gives a positive and specific immunostaining of LH-containing cells. Also shows reactivity to FSH-containing cells.
Species Reactivity
  • Canine
  • Human
  • Rat
  • Sheep
  • Feline
  • Ox
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene is a member of the glycoprotein hormone beta chain family and encodes the beta subunit of luteinizing hormone (LH). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.
Gene Symbol
  • LHB
  • hLHB
  • LSH-B
  • LH-B
  • LSH-beta
  • CGB4
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01229 # Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
SIZE: 141 amino acids; 15345 Da
SUBUNIT: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Pituitary gland.
DISEASE:SwissProt: P01229 # Defects in LHB are a cause of hypogonadism [MIM:152780]. Hypogonadism is characterized by infertility and pseudohermaphroditism.
SIMILARITY: SwissProt: P01229 ## Belongs to the glycoprotein hormones subunit beta family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Store at -20°C in undiluted aliqutos for up to 12 months. Avoid repeated freeze/thaw cycels.
Packaging Information
Material Size 500 µL
Transport Information
Supplemental Information
Specifications

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