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Rt X Ms CD31, clone 390 FITC-Conjugated

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货号：CBL1337F

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M FC, IHC R FITC Monoclonal Antibody
Description
Catalogue Number CBL1337F
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-PECAM-1 Antibody, clone 390, FITC conjugated
Alternate Names
  • CD31
Background Information PECAM-1 is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.
References
Product Information
Format FITC
Presentation Purified immunoglobulin conjugated to fluorescein. Liquid in in phosphate buffered saline containing 0.1% sodium azide.
Applications
Application This Anti-PECAM-1 Antibody, clone 390, FITC conjugated is validated for use in FC, IH for the detection of PECAM-1.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
Application Notes Flow Cytometry: = 1 μg per 10e6 cells in 100 μL volume

Immunohistochemistry: acteone fixed frozen tissue sections.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Mouse leukocyte cell line 32D
Clone 390
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rat
Specificity CBL1337 recognizes mouse PECAM-1, a 130-140 kDa type 1 integral membrane glycoprotein. This antibody has been shown to inhibit the aggregation of L cells transfected with a variant form of PECAM-1 (DeLisser, 1993).
Isotype IgG2aκ
Species Reactivity
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PECAM1
  • EndoCAM
  • GPIIA
  • PECAM-1
  • CD31
  • CD31/EndoCAM
UniProt Number
UniProt Summary FUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.
SIZE: 738 amino acids; 82536 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.
PTM: Phosphorylated on Ser and Tyr residues after cellular activation.
SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2°-8°C for up to 12 months from date of receipt. DO NOT FREEZE.
Packaging Information
Material Size 500 µg
Transport Information
Supplemental Information
Specifications

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